Anti h3s10p

Manufactured by Abcam

Anti-H3S10P is a lab equipment product that detects the phosphorylation of serine 10 on histone H3. This antibody can be used in various applications, such as Western blotting and immunocytochemistry, to study chromatin dynamics and cell cycle regulation.

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3 protocols using anti h3s10p

Antibody Procurement and Characterization

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The following antibodies were obtained commercially: anti-p53 (Santa Cruz Biotechnology); anti-p21, anti-MSK1, anti-MSK1 T581-P and anti-H3S10-P (Abcam); and anti-MSK1 S212-P (R&D Systems). All other antibodies were purchased from Sigma.

Ahn J., Lee J.G., Chin C., In S., Yang A., Park H.S., Kim J, & Park J.H. (2018). MSK1 functions as a transcriptional coactivator of p53 in the regulation of p21 gene expression. Experimental & Molecular Medicine, 50(10), 132.

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Antibody Immunoblotting for Protein Expression

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Antibodies were purchased from the following companies: anti-GLA from Santa Cruz; anti-PP1, Akt, phospho-PP1 at Thr³²⁰, phospho-Akt at Ser⁴⁷³, phospho-Akt at Thr³⁰⁸ from Cell Signaling Technology; anti-hnRNP A1 from Sigma; anti-hnRNP A2B1 from Acris; anti-SRp20 and SF2/ASF from Thermo Scientific; anti-H3K4me3, anti-H3K9me3, anti-H3K27me3, anti-H3K36me3, anti-H3K9me3, anti-H3K9A, anti-H3K9A, anti-H3K23A, anti-H3K27A, anti-H3S10P, anti-histone H3, anti-actin, and anti-HSP70 from Abcam; anti-p54nrb/NONO from Affinity Bioreagents; anti-PSIP1 from Bethyl Laboratories.

Chang W.H., Niu D.M., Lu C.Y., Lin S.Y., Liu T.C, & Chang J.G. (2017). Modulation the alternative splicing of GLA (IVS4+919G>A) in Fabry disease. PLoS ONE, 12(4), e0175929.

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Spindle Extraction and Immunoblotting

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The samples were electrophoresed on a SDS-PAGE gel, transferred on nitrocellulose membrane (Amersham) and immunoblotted with anti-MLL C (1:250, Bethyl), anti-WDR5 (1:1000,Bethyl), anti-RbBP5 (1:1000, Bethyl), anti-Kif2A (1:1000, Abcam), anti-GFP (1:1000, Invitrogen), anti-GAPDH (1:10000, Sigma), anti-GST(1:10000, Abcam), anti-H3S10P (abcam), and anti-MLL2 (1:250, Bethyl).
Proteins were detected with Licor-Biosciences imaging system as described previously (Tyagi et al., 2007) using IR Dye 800CW conjugated anti-mouse IgG( Licor Biosciences), IR Dye 680 LT conjugated anti-rabbit IgG ( Licor Biosciences).
Spindle Extraction U2OS cells were seeded in 150 x 25mm tissue culture plates. At 60-70% confluency cells were synchronized by thymidine-nocodazole block as described (Harper, 2005) . The cells were then harvested by mitotic shake off method. The spindle extraction was done as described (Sillje ´and Nigg, 2006) . The spindle extract was subjected to SDS-PAGE followed by immunoblotting.

Ali A., Veeranki S.N., Chinchole A, & Tyagi S. (2017). MLL/WDR5 Complex Regulates Kif2A Localization to Ensure Chromosome Congression and Proper Spindle Assembly during Mitosis. Developmental cell, 41(6).

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