ACY-241 (Citarinostat) and JQ1 were purchased from Selleck Chemicals (Houston, TX, USA). Powders were solubilized in DMSO (Sigma Chemical, St. Louis, MO, USA) to make 50 mM stocks. Antibodies against α-tubulin (sc-32293), c-Myc (sc-40), Snail (sc-28199), p-AKT (sc-7985-R), AKT (sc-8312), p65 (sc-8008), and TNF-α (sc-52746) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against GAPDH (AP0066) and p-p65 (BS4138) were from Bioworld Technology (Bloomington, MN, USA). Antibodies against MMP-2 (A2454) and MMP-9 (A0289) were from ABclonal Technology (Woburn, MA, USA). Antibodies against PARP (551024) and XIAP (610716) were from BD Biosciences (San Jose, CA, USA). Antibodies against caspase-3 (#9662), Bcl-xL (#2762), and MT1-MMP (#13130) were from Cell Signaling Technology (Danvers, MA, USA). Antibody against acetyl α-tubulin (T6793) was from Sigma-Aldrich (St. Louis, MO, USA). Antibody against HDAC6 was from Bethyl Laboratories (Montgomery, TX, USA).
Citarinostat
Manufactured by Selleck Chemicals
Sourced in United States
Citarinostat is a chemical compound used in laboratory research. It is a histone deacetylase (HDAC) inhibitor, a class of compounds that modulate the activity of HDAC enzymes. Citarinostat can be used to study the effects of HDAC inhibition on cellular processes and gene expression.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Tnf α sc 52746, by Santa Cruz Biotechnology (1 mentions)
Mt1 mmp, by Cell Signaling Technology (1 mentions)
Antibodies against gapdh ap0066 and p p65 bs4138, by Bioworld Technology (1 mentions)
Antibody against acetyl α tubulin t6793, by Merck Group (1 mentions)
Duvelisib, by Selleck Chemicals (1 mentions)
Copanlisib, by Selleck Chemicals (1 mentions)
Ricolinostat, by Selleck Chemicals (1 mentions)
Resminostat, by Selleck Chemicals (1 mentions)
Pictilisib, by Selleck Chemicals (1 mentions)
Trifluoric acid, by Merck Group (1 mentions)
293t cell, by American Type Culture Collection (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using citarinostat
1
Inhibition of HDAC6 and NF-κB Pathways
2
Cell Line Culturing and Chemical Inhibitor Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The HH, H9 and HUT78 cell lines were a generous gift from Dr Giandomenico Russo (Istituto Dermopatico dell'Immacolata, IDI–IRCCS, Rome, Italy). The SeAX cell line was a generous gift from Dr Katarzyna Izykowska (Institute of Human Genetics, Polish Academy of Science, Poznan, Poland). 293T cells were purchased from ATCC. The cells were maintained in RPMI-1640 medium (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum, L-glutamine (2 mM), penicillin (100 U/ml) and streptomycin (100 µg/ml). HDAC inhibitors (ricolinostat, citarinostat and resminostat), as well as PI3K inhibitors (duvelisib, copanlisib and pictilisib) were purchased from Selleckchem. Inhibitors were dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich; Merck KGaA) or 10% trifluoric acid (Sigma-Aldrich; Merck KGaA) in the case of copanlisib.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!