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Synergi 10 μ hydro rp 80a

Manufactured by Phenomenex
Sourced in United States

Synergi 10 μ Hydro-RP 80A is a reversed-phase high-performance liquid chromatography (HPLC) column produced by Phenomenex. The column features a particle size of 10 micrometers and a pore size of 80 angstroms, designed for the separation and analysis of a variety of organic compounds in aqueous environments.

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3 protocols using synergi 10 μ hydro rp 80a

1

Chromatographic Isolation of Gentianoside Compounds

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Column chromatography was performed using silica gel (70–230 mesh; Merck, Darmstadt, Germany). High-performance liquid chromatography was performed using the Agilent 1260 series system (Agilent Technologies, Santa Clara, CA, USA) with a C18 column (Phenomenex Synergi 10 μ Hydro-RP 80A, 10 μm, 4.6 mm × 250 mm, Torrance, CA, USA). Gentianae Scabrae Radix roots were purchased from a commercial herbal medicine market. A voucher specimen, P542, was deposited in the Natural Products Bank at the National Institute for Korean Medicine Development (NIKOM).
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2

Isolation and Characterization of Artemisia capillaris Compounds

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Artemisia capillaris Thunb was purchased from Korean medicine Omniherb (www.omnishop.co.kr). The P149 voucher specimen was deposited in the Natural Products Bank (NIKOM). Column chromatography (CC) was conducted using silica gel (Merck, Darmstadt, Germany). 13C Nuclear magnetic resonance (NMR) and 1H NMR spectra were analysed using JEOL ECX‐500 spectrometer (JEOL Ltd.). Agilent 1260 series (Agilent Technologies) with a C18 column (Phenomenex, United States of America Synergi 10 μ Hydro‐RP 80A, 10 μm, 4.6 mm × 250 mm) was used for high‐performance liquid chromatography (HPLC).
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3

Radiosynthesis of [18F]Compound 2

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The same methods described for the synthesis of [18F]1 were used to prepare [18F]2, except that the appropriate precursor 21 was used instead of precursor 11. HPLC purification conditions were slightly different: Phenomenex Synergi 10μ Hydro-RP 80A, 250×10 mm, 3.5% EtOH in 40 mM NH4OAc buffer, flow rate 4.0 mL/min, λ = 254 nm. In this system, [18F]2 was collected at Rt = 31–33 min.
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