The isolated neurons, adjusted to the concentration of 3×105/mL, were incubated in 10% FBS-containing DMEM (Gibco). After cultivation in 5% CO2 at 37℃ for 1 h, the neurons were transfected with si-Zfas1-1 (5′-CUGGCUGAACCAGUUCCACAAGGUU-3′; GenePharma, Shanghai, China), si-Zfas1-2 (5'-CCCTGTGCTTTCATGAAAGTGAAGA-3′; GenePharma), si-NC (5′-CCAAAACCAGGCUUUGAUUGA-3′; GenePharma), pcDNA3.1-Zfas1 (GenePharma) or pcDNA3.1 (GenePharma) for 48 h, with the assistance of Lipofectamine 2000 transfection reagent (Invitrogen, Carlsbad, CA, USA).
Si zfas1 2
Manufactured by GenePharma
Sourced in China
The Si-Zfas1-2 is a laboratory equipment product designed for research purposes. It serves as a tool for the quantification and analysis of the Zfas1 gene expression. The core function of this product is to facilitate the measurement and detection of the Zfas1 gene in research samples.
Automatically generated - may contain errors
Lab products found in correlation
Si zfas1 1, by GenePharma (2 mentions)
Pcdna3, by GenePharma (1 mentions)
Si nc, by GenePharma (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Pcdna3.1 zfas1, by GenePharma (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Inh nc, by GenePharma (1 mentions)
2 protocols using si zfas1 2
1
Modulating Zfas1 in Cultured Neurons
2
Cholangiocarcinoma Cell Line Manipulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cholangiocarcinoma cell lines (CCLP‐1, RBE, QBC939 and HuCCT1) and normal control cell line HIBEC were preserved in the laboratory as previously described.28 Cells were cultured in 10% foetal bovine serum (FBS) loaded RPMI‐1640 medium (Invitrogen Life Technologies) and 100 μg/mL penicillin/streptomycin supplemented at 37°C with 5% CO2 in a humidified incubator. Small interfering RNAs targeting ZFAS1 (si‐ZFAS1‐1 and si‐ZFAS1‐2), USF1 (si‐USF1), negative controls (si‐ZFAS1‐NC, si‐USF1‐NC) were purchased from Shanghai GenePharma company, and so did miR‐296‐5p inhibitor and corresponding negative control (inh‐miR‐296‐5p, inh‐NC). Lipofectamine 2000 (Thermo Fisher Scientific, Inc) was used for transfection according to the manufacturer's instructions. The transfected cells were harvested 48 hours after transfection.
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