E faecalis

Manufactured by Merck Group

Sourced in Germany

E. faecalis is a species of Gram-positive bacteria commonly found in the human gastrointestinal tract. This bacterium can be used in laboratory settings for various research and testing purposes.

Automatically generated - may contain errors

Lab products found in correlation

C albicans, by Microbiologics (1 mentions) K pneumoniae, by Microbiologics (1 mentions) A brasiliensis, by Microbiologics (1 mentions) K pneumoniae, by Merck Group (1 mentions) P aeruginosa, by Microbiologics (1 mentions) B subtilis, by Merck Group (1 mentions) S aureus, by Merck Group (1 mentions) P aeruginosa, by Merck Group (1 mentions) S aureus, by American Type Culture Collection (1 mentions) E faecalis, by American Type Culture Collection (1 mentions) Rpmi 1640, by Merck Group (1 mentions) E faecalis atcc 29212, by American Type Culture Collection (1 mentions)

2 protocols using e faecalis

Standardized Microbial Inoculum Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

S. aureus (American Type Culture Collection, ATCC 6538), E. faecalis (ATCC 29212), B. subtilis spizizenii (ATCC 6633), E. cloacae (ATCC 35030), K. pneumoniae (ATCC 10031), A. brasiliensis (ATCC 16404), P. aeruginosa (ATCC 9027) and C. albicans (ATCC 10231) were obtained from Microbiologics (Minnesota, USA). F. solani (Deutsche Sammlung von Mikroorganismen, DSM 1164) was supplied by Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Germany.
Inocula of S. aureus, E. faecalis, B. subtilis, E. cloacae, A. brasiliensis, P. aeruginosa, C. albicans, K. pneumoniae and F. solani were prepared from lyophilised pellets according to the manufacturer’s protocols or from fresh culture according to McFarland standards to obtain 1.0×10⁷ colony forming units (CFU/mL in physiological solution (0.9% (w/v) NaCl); Merck-Sigma-Aldrich, Darmstadt, Germany.
Tryptone soya broth (20 mL; Bioculturalab, Italy) was used as growth control for C. albicans, A. brasiliensis and F. solani, and thioglycolate medium (20 mL; Bioculturalab) was used as growth control for S. aureus, B. subtilis, P. aeruginosa and K. pneumoniae. Nutrient broth (Nutrient Broth, 20 mL; BioLife, Italy) medium was used as growth control for E. faecalis and E. cloacae.

Giurgola L., Gatto C., Honisch C., Rossi O., Ragazzi E, & D'Amato Tothova J. (2021). Killing efficacy of a new hypothermic corneal storage medium against the micro-organisms frequently found in human donor cornea intended for transplantation. BMJ Open Ophthalmology, 6(1), e000833.

+ Open protocol

+ Expand

Cultivation of C. albicans and E. faecalis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The strain of C. albicans (YP0037) used in this study was obtained from the Pathogenic Yeast Collection of FMUC, University of Coimbra. E. faecalis (ATCC29212) was purchased from the American Type Culture Collection (ATCC). Microrganisms were stored at -80°C in 25% glycerol. When needed, pre-cultures were prepared by defrozen microbial cells in appropriate media, brain-heart infusion (BHI) for E. faecalis and YPD (0.5% yeast extract, 1% bacto-peptone, and 2% glucose) for C. albicans. For C. albicans growth it was used YPD broth. E. faecalis growth and biofilm formation was obtained in BHI liquid medium (Difco, Detroit, MI, USA). C. albicans biofilms and mixed biofilms consisting of C. albicans with E. faecalis were obtained in RPMI 1640 (Roswell Park Memorial Institute) medium (R8755, Sigma–Aldrich^®).

Diogo P., Fernandes C., Caramelo F., Mota M., Miranda I.M., Faustino M.A., Neves M.G., Uliana M.P., de Oliveira K.T., Santos J.M, & Gonçalves T. (2017). Antimicrobial Photodynamic Therapy against Endodontic Enterococcus faecalis and Candida albicans Mono and Mixed Biofilms in the Presence of Photosensitizers: A Comparative Study with Classical Endodontic Irrigants. Frontiers in Microbiology, 8, 498.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!