Glutathione assay kit

Unless otherwise stated, all chemicals were purchased from Sigma-Aldrich Chemie (Schnelldorf, Germany). Disulfiram was purchased via MCE (Shanghai, China). Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12) and phosphate-buffered saline (PBS) were purchased via Gibco (Shanghai, China). Fetal bovine serum was purchased via HyClone (Shanghai, China). Pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin were purchased from Sansheng (Ningbo, China). The Reactive Oxygen Species Assay Kit was purchased via Beyotime (Shanghai, China). The Glutathione Assay Kit was purchased via Thermo Fisher Scientific (Shanghai, China). Kits for total RNA extraction, reverse transcription and fluorescence quantitation kit were purchased via Tiangen Biotech (Beijing, China). The HE Staining Kit was purchased via Solarbio (Beijing, China). The primer synthesis was conducted by Tsingke Biotech (Beijing, China). The sequences were searched in the NCBI BLAST database.

Dextran sodium sulfate (DSS) was purchased from MP Biomedicals (Santa Ana, CA, USA) and 5-aminosalicylic acid (5-ASA), hematoxylin, and eosin (H&E) solutions were obtained from Sigma Aldrich (St Louis, MO, USA). ELISA kits for myeloperoxidase (MPO) activity and for mouse tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and IL-1β were purchased from Thermo Fisher Scientific (Waltham, MA, USA) and eBioscience (San Diego, CA, USA), respectively. Superoxide Dismutase Activity kit, Malondialdehyde (MDA) assay kit, Catalase Activity Kit, and Glutathione Assay Kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA) and Abcam (Cambridge, UK). RIPA lysis buffer and phosphatase were obtained from Millipore (Darmstadt, Germany) and protease inhibitor cocktails were obtained from Roche (Basel, Swiss). BCA protein quantification kit was purchased from Thermo Fisher Scientific and anti-ZO-1, anti-occludin, and anti-F4/80 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).