Western blotting analysis was performed according to the standard protocol described previously [22 (link)].
The samples were subjected to SDS-PAGE and transferred onto a polyvinylidene fluoride membrane. The primary antibodies used in this study were as follows: anti-HAb18G (1:4,000 [24 (link)], prepared by our lab), rabbit-anti-β-catenin (1:500, Santa Cruz), rabbit-anti-APC (1:500, Boster), rabbit-anti-VEGF (1:400, Boster), rabbit-anti-NF-κB p65 (1:400, Boster), and an anti-α-tubulin antibody as a loading control. The secondary antibodies used were either goat anti-mouse or goat anti-rabbit IgG (PIERCE), depending on the primary antibody used.
The samples were subjected to SDS-PAGE and transferred onto a polyvinylidene fluoride membrane. The primary antibodies used in this study were as follows: anti-HAb18G (1:4,000 [24 (link)], prepared by our lab), rabbit-anti-β-catenin (1:500, Santa Cruz), rabbit-anti-APC (1:500, Boster), rabbit-anti-VEGF (1:400, Boster), rabbit-anti-NF-κB p65 (1:400, Boster), and an anti-α-tubulin antibody as a loading control. The secondary antibodies used were either goat anti-mouse or goat anti-rabbit IgG (PIERCE), depending on the primary antibody used.