Similar superovulation protocols were utilized for all couples. Patients were treated with gonadotropins daily (Follistim; Merck, Kenilworth, NJ, USA; Gonal-F; EMD Serono, Geneva, Switzerland; and/or Menopur; Ferring Pharmaceuticals Inc, Parsippany, NJ, USA). GnRH-antagonist (Ganirelix acetate; Merck, Kenilworth, NJ, USA; or Cetrotide; EMD Serono In. Rockland, MA, USA) was administered to prevent precocious ovulation. Human chorionic gonadotropin (hCG) (Pregnyl Merck) was administered to trigger final oocyte maturation when the two leading follicles reached a diameter of ≥17 mm. Ultrasound-guided transvaginal oocyte retrieval was performed under anesthesia 35–37 h after hCG trigger. A period of 2 h after retrieval, oocyte-cumulus-complexes were denuded by 40 IU/ml recombinant human hyaluronidase (ICSI Cumulase; Cooper Surgical, Inc, Trumbull, CT). Oocytes were then incubated 1–2 h prior to ICSI.
Icsi cumulase
Manufactured by CooperSurgical
Sourced in United States
ICSI Cumulase is a laboratory product designed for use in assisted reproductive technology procedures. It contains a purified enzyme solution that helps in the separation of cumulus cells from the oocyte (egg) during the intracytoplasmic sperm injection (ICSI) process. The product is intended for professional use in appropriate clinical settings.
Automatically generated - may contain errors
3 protocols using icsi cumulase
1
Superovulation and Oocyte Retrieval Protocol
2
Standardized Ovarian Stimulation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stimulation protocols were similar between female patients with male partners in the normal, moderate, and severe SCF groups. Patients were treated daily with gonadotropins (Ovidrel [Merck KGaA, Darmstadt, Germany], Gonal-F [EMD-Serono, Geneva, Switzerland], and/or Menopur [Ferring Pharmaceuticals Inc., Parsippany, NJ]). A gonadotropin-releasing hormone antagonist (Ganirelix acetate [Merck, Kenilworth, NJ] or Cetrotide [EMD-Serono Inc., Rockland, MA]) was used to inhibit premature luteinizing hormone surges in controlled ovarian stimulation to prevent precocious ovulation. When at least 2 follicles reached a diameter of ≥ 17 mm, human chorionic gonadotropin (hCG) (Pregnyl Merck) was administered to trigger ovulation. Oocyte retrieval was performed under sedation by a transvaginal sonography-guided technique 35–37 hours after hCG administration. Two hours after oocyte collection, cumulus cells were denudated by 40 IU/mL of recombinant human hyaluronidase (ICSI Cumulase; Cooper Surgical, Inc., Trumbull, CT) (11 (link)). Cleaned oocytes were then incubated 1–2 hours before ICSI, which has been previously described (12 (link)).
3
Oocyte Retrieval and Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oocyte retrieval was performed 35.5 h after hCG administration, with transvaginal ultrasound‐guided aspiration. Oocytes were collected from the follicular fluid and washed in freshly equilibrated human tubal fluid medium with HEPES (Multipurpose Handling Medium; Irvine Scientific, CA, USA). They were then incubated in an insemination medium (NAKA medical inc, Tokyo, Japan) covered with mineral oil (Light Mineral Oil for Embryo Culture; Irvine, Cal., USA) for 2–4 h with 6.5% CO2/5% O2/88.5% N2 at 37°C in an incubator (MINC™, COOK MEDICAL INC, IN, USA).
The oocytes were denuded of the cumulus oophorus by a brief exposure to 40 IU/mL hyaluronidase (ICSI Cumulase; Cooper surgical, Trumbull, USA), and a pipette (STRIPPER; Cooper surgical, Trumbull, USA) was used to mechanically remove the oocytes from the surrounding cumulus cells.
Denuded oocytes were incubated in single‐step medium (ONE STEP Medium; NAKA medical inc, Tokyo, Japan) covered with mineral oil.
The oocytes were denuded of the cumulus oophorus by a brief exposure to 40 IU/mL hyaluronidase (ICSI Cumulase; Cooper surgical, Trumbull, USA), and a pipette (STRIPPER; Cooper surgical, Trumbull, USA) was used to mechanically remove the oocytes from the surrounding cumulus cells.
Denuded oocytes were incubated in single‐step medium (ONE STEP Medium; NAKA medical inc, Tokyo, Japan) covered with mineral oil.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!