Olaparib (AZD2281; Selleckchem, Houston, TX, USA) and talazoparib (T6253; TargetMol, Wellesley Hills, MA, USA) were resuspended in DMSO at a concentration of 100 mM or 200 mM, respectively.
Talazoparib
Manufactured by Targetmol
Sourced in United States
Talazoparib is a lab equipment product. It is a poly(ADP-ribose) polymerase (PARP) inhibitor used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Olaparib, by Selleck Chemicals (3 mentions)
Niraparib, by Targetmol (2 mentions)
Veliparib, by Targetmol (2 mentions)
Olaparib, by Targetmol (2 mentions)
Cell counting kit 8 cck 8, by Yeasen (2 mentions)
Sgc0946, by Targetmol (1 mentions)
Niraparib, by Selleck Chemicals (1 mentions)
Rucaparib, by Selleck Chemicals (1 mentions)
Anti ubiquitin, by Cell Signaling Technology (1 mentions)
Anti rna polymerase 2, by Abcam (1 mentions)
Anti rna polymerase 2 phospho s2, by Abcam (1 mentions)
Anti p65, by Cell Signaling Technology (1 mentions)
Anti phospho p65, by Cell Signaling Technology (1 mentions)
Anti poly adp ribose, by Merck Group (1 mentions)
Anti ki67, by Cell Signaling Technology (1 mentions)
Anti puromycin, by Merck Group (1 mentions)
Anti parp1, by Cell Signaling Technology (1 mentions)
Anti p21, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
5 protocols using talazoparib
1
PARP Inhibitor Stock Preparation
2
Resuspension of PARP Inhibitors
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Olaparib (AZD2281; Selleckchem, Houston, TX, USA) and talazoparib (T6253; TargetMol, Wellesley Hills, MA, USA) were resuspended in DMSO at a concentration of 100 mM or 200 mM, respectively.
3
Synergistic Effects of DOT1L and PARP Inhibitors
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DOT1L inhibitor SGC0946 and PARP inhibitors Olaparib, Niraparib, Veliparib, and Talazoparib were all purchased from TargetMol. For drug synergy studies, ovarian cancer cells (4000/well) were seeded in 96-well plates for overnight incubation and treated with different doses of inhibitors for 5 days. Cell viability was evaluated by measuring the 450 nm absorbance with the Cell Counting Kit-8 (CCK-8) (YEASEN, Shanghai, China). Each concentration was tested in triplicate. The IC50 value was calculated and performed in GraphPad Prism v8.0. Drug synergistic effects were calculated based on the CompuSyn software or SynergyFinder. CI < 1 indicated synergism, CI = 1 indicated additive effects, and CI > 1 indicated antagonism.
4
Determining PARP Inhibitor IC50 Values
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To determine 50% inhibitory concentration (IC50) values of Olaparib (AZD2281; TargetMol, USA), Niraparib (MK-4827; TargetMol, USA), Veliparib (T2591; TargetMol, USA), and Talazoparib (T6523, TargetMol, USA), we measured the cell proliferation rate using Cell Counting Kit-8 (CCK-8) (YEASEN, Shanghai, China). IC50 values were analyzed using GraphPad Prism Version 8.0.
5
Investigating Epigenetic and Transcriptional Regulation
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The following primary antibodies were used in the research: anti-Ki67, anti-phospho-γH2A, anti-DIMT1, anti-GAPDH, anti-NELF-E, anti-PARP1, anti-p65, anti-phospho-p65, anti-ubiquitin, anti-p21, (Cell Signaling Technology, USA). Anti-puromycin, anti-poly-ADP-ribose, (Merck Millipore, USA). Anti-RNA polymerase II, Anti-RNA polymerase II (phospho-S5), Anti-RNA polymerase II (phospho-S2), anti-RNF146, (Abcam, USA). anti-PARP1 (Active Motif, USA). Niraparib, PJ34, olaparib, and rucaparib were purchased from Selleck Chemicals (Houston, USA). Talazoparib was purchased from TargetMol (Boston, USA). 5,6-Dichlorobenzimidazole 1-β-D-ribofuranosylbenzimidazole (DRB) was purchased from APExBIO (Houston, USA).
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