Bp 2000 series 2 instrument

Blood pressure was measured on the 7th, 14th, and 21st day after the start of treatment. Measurements of SBP and DBP were repeated three times for each animal, and the mean value was recorded. The animals were allowed to rest for at least 15 minutes at 30°C before blood pressure was measured. Blood pressure was measured by a tail-cuff using a BP-2000 series II instrument (Visitech Systems, USA).

Eight-to 12-week-old CD-1 female mice weighing 25–35 g from the Charles River (Beijing, China) were mated with age-matched male mice. Pregnant mice were randomly assigned to three groups [gestation day 14.5, adenovirus (Ad)-control (Ctrl), n = 5; Ad-TAGLN2, n = 5; and gestational day 18.5, Ad-Ctrl, n = 5]. Adenovirus carrying shRNA targeting mouse TAGLN2 was purchased from Genechem (Shanghai, China). Age-matched nonpregnant (NP) mice were divided into two groups (Ad-Ctrl, n = 5; Ad-TAGLN2, n = 5). The presence of a vaginal plug was considered gestational day 0.5. On gestational day 8.5, the pregnant mice were injected with Ad-Ctrl or Ad-TAGLN2 (2 × 10⁹ PFU, 100 μL) through the tail vein. systolic blood pressure was monitored by a tail cuff photoplethysmography BP-2000 Series II instrument (Visitech Systems, Apex, NC, United States) daily from gestational day 6.5–18.5. The mice were euthanized on gestational day 18.5 to collect placentas to examine the expression of TAGLN2 and weights of fetal and placental.