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Fplc gel filtration column

Manufactured by GE Healthcare
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The FPLC gel filtration column is a laboratory equipment used for size-based separation of biomolecules such as proteins, enzymes, and other macromolecules. It operates on the principle of size exclusion chromatography, allowing the separation of molecules based on their molecular weight or size as they pass through a porous stationary phase.

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Lab products found in correlation

Alcohol dehydrogenase, by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Apoferritin, by Merck Group (2 mentions) Thyroglobulin, by Merck Group (2 mentions) Carbonic anhydrase, by Merck Group (2 mentions) β amylase, by Merck Group (2 mentions) Superose 6 hr 10 30 fast protein liquid chromatography, by GE Healthcare (2 mentions)

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FPLC columns (9 citations)

2 protocols using fplc gel filtration column

1

Purification of HeLa Cell Complexes

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Four milligrams of HeLa endogenous extract was injected onto a Superose 6 HR 10/30 fast protein liquid chromatography (FPLC) gel filtration column (GE Healthcare), equilibrated with 20mM Tris-HCl (pH 7.5), 200mM NaCl, 12.5% glycerol, 0.2% Nonidet P-40, 1mM EDTA, 1mM EGTA and 1mM DTT, and eluted at a flow rate of 0.25ml/min with the same buffer. Proteins were collected in 250µl fractions, precipitated with 10% trichloroacetic acid (TCA), and subjected to Western blotting. Gel filtration columns were calibrated with the following molecular mass markers: thyroglobulin (669kDa), apoferritin (443kDa), β-amylase (200kDa), alcohol dehydrogenase (150kDa), bovine serum albumin (66kDa), and carbonic anhydrase (29kDa) (Sigma Aldrich).
Cianfanelli V., Fuoco C., Lorente M., Salazar M., Quondamatteo F., Gherardini P.F., De Zio D., Nazio F., Antonioli M., D’Orazio M., Skobo T., Bordi M., Rohde M., Dalla Valle L., Helmer-Citterich M., Gretzmeier C., Dengjel J., Fimia G.M., Piacentini M., Di Bartolomeo S., Velasco G, & Cecconi F. (2014). AMBRA1 links autophagy to cell proliferation and tumorigenesis by promoting c-MYC dephosphorylation and degradation. Nature cell biology, 17(1), 20-30.
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2

Purification of HeLa Cell Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Four milligrams of HeLa endogenous extract was injected onto a Superose 6 HR 10/30 fast protein liquid chromatography (FPLC) gel filtration column (GE Healthcare), equilibrated with 20mM Tris-HCl (pH 7.5), 200mM NaCl, 12.5% glycerol, 0.2% Nonidet P-40, 1mM EDTA, 1mM EGTA and 1mM DTT, and eluted at a flow rate of 0.25ml/min with the same buffer. Proteins were collected in 250µl fractions, precipitated with 10% trichloroacetic acid (TCA), and subjected to Western blotting. Gel filtration columns were calibrated with the following molecular mass markers: thyroglobulin (669kDa), apoferritin (443kDa), β-amylase (200kDa), alcohol dehydrogenase (150kDa), bovine serum albumin (66kDa), and carbonic anhydrase (29kDa) (Sigma Aldrich).
Cianfanelli V., Fuoco C., Lorente M., Salazar M., Quondamatteo F., Gherardini P.F., De Zio D., Nazio F., Antonioli M., D’Orazio M., Skobo T., Bordi M., Rohde M., Dalla Valle L., Helmer-Citterich M., Gretzmeier C., Dengjel J., Fimia G.M., Piacentini M., Di Bartolomeo S., Velasco G, & Cecconi F. (2014). AMBRA1 links autophagy to cell proliferation and tumorigenesis by promoting c-MYC dephosphorylation and degradation. Nature cell biology, 17(1), 20-30.
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