B 27 culture mediums

Chemical compounds to make saline solutions, as well as isoprenaline hydrochloride were purchased from Sigma (Sigma-Aldrich, St Louise, MO, USA). Amphotericin B was acquired from Fisher Scientific (Pittsburgh, PA, USA). Every experimental day, a stock solution of isoprenaline hydrochloride was prepared in deionized water and Amphotericin B in DMSO. The inhibitors CHIR 99021 were acquired from Selleckchem (Houston, TX, USA) and IWP2 from TOCRIS (Minneapolis, MN, USA), E8, RPMI and B-27 culture mediums were purchased from Life Technologies/GIBCO (Grand Island, NY, USA). The novel and potent Ca_V1.3-highly selective antagonist, 1-(3-chlorophenethyl)-3-cyclopentylpyrimidine-2,4,6-(1H,3H,5H)-trione [30 (link)], was purchased from Calbiochem-EMD chemicals (San Diego, CA, USA).

Chemical as well as isoprenaline hydrochloride were purchased from Sigma (Sigma-Aldrich, St Louise, MO, USA). Amphotericin B was acquired from Fisher Scientific (Pittsburgh, PA, USA). Stock solution of isoprenaline hydrochloride was prepared daily in deionized water and Amphotericin B in DMSO. The inhibitor CHIR99021 was acquired from Selleckchem (Houston, TX, USA) and IWR1 from TOCRIS (Minneapolis, MN, USA), StemFlex, RPMI and B-27 culture mediums were purchased from ThermoFisher Scientific/GIBCO (Grand Island, NY, USA).