Rabbit anti map2

The following antibodies were used: mouse anti-β-galactosidase (Promega, Mannheim, Germany), mouse anti-parvalbumin, rabbit anti-SK2 (Sigma-Aldrich, Munich, Germany), rabbit anti-GFAP (Dako, Glostrup, Denmark), rabbit anti-parvalbumin, mouse anti-GAD67 (Merck Millipore, Darmstadt, Germany), rabbit anti-Arc, rabbit anti-VGLUT2, rabbit anti-MAP2 [35 (link)] (Synaptic Systems, Göttingen, Germany), rabbit anti-Iba1 (Wako Chemicals GmbH, Neuss, Germany), rabbit anti-BDNF (Santa Cruz Biotechnology, Heidelberg, Germany), rabbit anti-BK (Alomone Labs, Jerusalem, Israel), rabbit anti-CtBP2/RIBEYE (Cell Applications, San Diego, CA, USA), and rabbit anti-KCNQ4 [36 (link)].

Sagittal brain sections were obtained on a Leica VT1200S vibratome (50-µm-thick sections). For immunohistochemical analysis, series of brain slices were randomly made up of one section from every ninth. Slices were initially pre-incubated in phosphate buffer with 1% Triton X-100 and 1% bovine serum albumin, and dual immunohistochemistry was then performed as described previously^{23 (link)}, using the following primary antibodies: rabbit anti-RFP (Millipore, 1:2000); mouse anti-Cy5 (Abcam, 1:500); goat anti-Doublecortin (DCX) (Santa Cruz, 1:500); rabbit anti-Fractin (a fragment of actin cleaved by caspase 3) (BD Biosciences, 1:500); mouse anti-BrdU/IdU (BD Biosciences, 1:500); rabbit anti-MAP-2 (Synaptic Systems, 1:500); and rabbit anti-NeuN (Millipore, 1:1000). To detect the binding of primary antibodies, Alexa-488 donkey anti-mouse and Alexa-555 donkey anti-rabbit (Invitrogen, 1:1000) secondary antibodies were used. All the sections were counterstained for 10 min with DAPI (Merck, 1:5000) in order to label nuclei.