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2 protocols using potassium bichromate k2cr2o7

1

Antioxidant Activity Measurement Methods

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The 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH), 2,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), potassium persulfate (K2S2O8), butylate-hydroxytoluene, phenazine methosulphate, 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), diclofenac sodium, butylated hydroxytoluene (BHT), 2,2′-Azobis(2-methylpropionamidine) dihydrochloride (AAPH), fluorescein sodium salt, sodium phosphate dibasic (Na2HPO4), potassium phosphate monobasic (KH2PO4), 2,4,6-Tris(2-pyridyl)-S-triazine (TPTZ), iron sulphate heptahydrate, sodium acetate, sodium carbonate, vanillin, Folin-Ciocalteu reagent, β-carotene, linoleic acid, tween 80, ferrozine, iron (II) chloride, EDTA, fatty free BSA, trypsin, tris-HCl, casein, potassium bichromate (K2Cr2O7) were purchased from Sigma-Aldrich (MSt. Louis, MO, USA). Methanol, acetonitrile, glacial acetic acid and phosphoric acid were HPLC grade and purchased from Merck (Darmstadt, Germany). Reference compounds were HPLC grade and purchased from Extrasynthese (Genay, France).
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2

Starch Azure Amylase Assay

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Starch azure, porcine pancreatic amylase, calcium chloride, acetic acid, hydrochloric acid, acetone, dimethyl sulfoxide (DMSO), dichloromethane (CH2Cl2), and potassium bichromate (K2Cr2O7) were purchased from Sigma Aldrich (Milan, Italy).
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