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2 protocols using β cedrene

1

Authentic Chemical Standards Protocol

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Authentic chemical standards of 16-hexadecanolide (97%), benzaldehyde (99%), 3-octanone (98%), 3-octanol (99%), limonene (97%), decanal (98%), undecanal (97%), undecane (99%), dodecane (99%), tridecane (99%), tetradecane (99%), pentadecane (98%), hexadecane (98.5%), heptadecane (98.5%), β-caryophyllene (98%), β-cedrene (95%), geranylacetone (97%), and the solvent n-hexane (97% redistilled) were purchased from Sigma-Aldrich. (E)-2-Octen-1-ol (98%) was purchased from Alfa Aesar. 1-Octen-3-ol, octanal, nonanal, 2-ethylhexan-1-ol, phenylacetaldehyde, phenylethanol, and β-bisabolene were donated by Dr. Jeffrey Aldrich (Agricultural Research Service – USDA). β-Sesquiphellandrene was purified from ginger oil and α-himachalene was purified from Cedrus atlantica oil.
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2

Characterization of Virginia Cedarwood Oil

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Virginia cedarwood oil was obtained from Texarome, Inc. (Leakey, TX) in a single lot. The chemical was identified as cedarwood oil using infrared (IR) and proton nuclear magnetic resonance (NMR) spectroscopy, and the purity was determined based on a chromatography profile of the major components obtained by gas chromatography (GC) with flame ionization detection (FID). For purity, five compounds (α-cedrene, β-cedrene, thujopsene, cuparene, and cedrol) were selected as marker compounds and quantitated using standards obtained from Sigma-Aldrich Corporation (St. Louis, MO). Weight percent concentrations of the five selected marker compounds were determined to be α-cedrene, 27.01%; β-cedrene, 6.05%; thujopsene, 19.64%; cuparene, 0.96%; and cedrol, 21.83%. Ethanol (95%; USP Grade) was obtained from Spectrum Chemicals & Laboratory Products (Gardena, CA) in a single lot. The cedarwood oil dose formulations were stable under simulated animal room conditions for up to 3 hours and for at least 42 days when stored in amber glass containers with Teflon®-lined lids at room temperature.
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