Hearts were fixed in buffered formalin solution, embedded in paraffin, and cut into 5 μm sections. Sections were stained with haematoxylin-eosin or used for the detection of T. cruzi antigens by means of the immunoperoxidase method. For the latter use, sections were deparaffinised and incubated with polyclonal anti-T. cruzi antiserum obtained from rabbits immunized with T. cruzi whole homogenate. Biotinylated goat anti–mouse/rabbit IgG and Duet Strepto ABComplex (Dako, Denmark) were used as a secondary antibody and for amplification, respectively. Diaminobenzadine (SIGMA Chemical Co, USA) was used as chromogen. preimmune rabbit serum was used as a negative control [17 (link)].
Biotinylated goat anti mouse rabbit igg
Manufactured by Agilent Technologies
Sourced in Denmark
Biotinylated goat anti–mouse/rabbit IgG is a secondary antibody conjugated with biotin. It is designed to detect and bind to mouse or rabbit primary antibodies, enabling their visualization or further application.
Automatically generated - may contain errors
2 protocols using biotinylated goat anti mouse rabbit igg
1
Immunohistochemical Detection of T. cruzi
2
Calretinin Immunohistochemistry in Ovarian Samples
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The immunohistochemical study for calretinin was focused on the ovarian sections of mothers and their offspring of 21 days old. Briefly, five-µm sections from the ovaries were mounted on lysine-coated slides (Surgipath, Richmond, IL). The sections were deparaffinized by xylene, graded ethanol, and water, then digested with a mixture of proteinase K (DAKO Corporation, Carpinteria, CA) and protease (0.1%, Sigma, St. Louis, MO) for 10 minutes. The sections were then incubated with a polyclonal rabbit anticalretinin antibody (1: 50, ZYMED Laboratories Inc, San Francisco, CA) at room temperature for 30 minutes. After that the sections were washed with PBS, followed by incubation in the secondary antibody (biotinylated goat anti-mouse/rabbit IgG, DAKO) for 20 minutes and then immersed in peroxidase-conjugated avidin (DAKO) at 25 0 C for 20 min. The reaction product was detected with 3, 3diaminobenzidine chromagen (DAKO). The sections were counterstained with 0.1% hematoxylin (Cao et al. 2001) (link).
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