Dynapro ms x dls detector

DLS was used to determine the apparent hydrodynamic radius (R_H) and polydispersity (Pd) of PHA nanoparticle suspensions as well as to characterize the effect of surfactant components on the nanomaterial size distribution. To that end, a DynaPro MS/X DLS detector equipped with an 824.7 nm-laser (Wyatt Technology, Santa Bárbara, CA, USA) was used. Briefly, the nanomaterial (1 mg/mL), alone or incubated for 10 min with NS, EO, PL, or DPPC multilamellar suspensions, was diluted 100-fold with milliQ water filtered 10 times with filters of 0.22 μm (Q-Pod, Merck, Darmstadt, Germany) and the hydrodynamic radius of the different components of the sample was calculated by the Stokes–Einstein equation (Equation (1)): $R_{\mathrm{H}}=\frac{k_{\mathrm{B}}T}{6\mathsf{\pi }\eta D}$
where D is the translational diffusion coefficient, k_B the Boltzman constant, T the temperature, and η the viscosity. The same surfactant suspensions analyzed by DLS were exposed to the presence of NPs.
Polydispersity values smaller than 15% were considered to correspond to monodisperse samples.

In order to characterize the OE samples after sonication, the hydrodynamic radius (R_H) of aqueous suspensions in the presence or the absence of 1% rhSP-D by mass were determined using a DynaPro MS/X DLS detector equipped with a 824.7 nm-laser (Wyatt Inc). R_H was calculated by the Stokes-Einstein equation (Equation 1):
where D is the translational diffusion coefficient, k_B the Boltzman constant, T the temperature, and η the viscosity. Water used to dilute the samples was 10 times filtered using filters of 0.22 μm (Q-Pod, Merck). Polydispersity values smaller than 15% were considered to correspond to monodisperse samples.