Separation was carried out on a LiChrospher®CN column (125 × 4.0 mm, 5 μm) from Merck. The column was housed in a column heater set at 22 °C. The mobile phase consisted of 0.1% formic acid, 10 mM ammonium formate and acetonitrile (55 : 15 : 30, v/v/v). The flow rate of the mobile phase was 1.4 mL min−1. Chromatography was performed with a model 306 pump with a loop Rheodyne (20 μL) and a model UV170 detector from Gilson (Middleton, USA) set at 228 nm for quantitative measurements, and additively at 254 and 290 nm to detect degradation products. The system was controlled by Omnic software from Gilson.
Model uv170 detector
Manufactured by Gilson
Sourced in United States
The Gilson Model UV170 detector is a compact and reliable spectrophotometric detector designed for analytical applications. It measures the absorption of ultraviolet and visible light by samples passing through a flow cell, providing accurate and consistent data for HPLC, LC, and other liquid chromatography systems. The UV170 detector features a wide wavelength range, adjustable sensitivity, and easy-to-use interface for seamless integration into analytical workflows.
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Lab products found in correlation
3 protocols using model uv170 detector
1
HPLC Analysis of Drug Compounds
2
HPLC Analysis of Organic Compounds
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Analysis was performed with a model 306 pump with a loop Rheodyne (20 µL) and a model UV170 detector controlled by Omnic software from Gilson (Middleton, WI, USA). Separation was carried out on a LiChrospher®CN column (125 × 4.0 mm, 5 µm) from Merck. The column was housed in a column heater set at 25 °C. The mobile phase consisted of water, 0.02 M tetrabutylammonium hydrogen sulfate and acetonitrile (20:65:15, v/v/v) adjusted to pH 3.5 with 0.5 M sulphuric acid. The flow rate of the mobile phase was 1.4 ml mL−1 while the detection was done at 235 nm.
3
Chromatographic Validation Protocol
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Chromatography was performed with a model 306 pump with a loop Rheodyne (20 μl) and a model UV170 detector controlled by OMNIC software (all from Gilson Inc., Middleton, WI, USA). The columns were housed in a column heater set at 25°C. The developed methods were validated according to the ICH and FDA guidelines for their specificity, linearity, sensitivity, accuracy, precision, and robustness [27 , 28 ].
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