Annexin 5 and pi apoptosis kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Annexin-V and PI apoptosis kit is a laboratory tool used to detect and quantify apoptosis, a type of programmed cell death. The kit utilizes Annexin-V, a protein that binds to phosphatidylserine, and propidium iodide (PI), a dye that stains the nucleic acids of cells with compromised membranes. This combination allows for the identification of cells in different stages of apoptosis.

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Close spelling variants of this product

Annexin V-FITC and PI apoptosis detection kit Dead Cell Apoptosis Kit with Annexin V-fluorescein (FITC) and PI (V13242), Dead Cell Apoptosis Kit with Annexin V Alexa Fluor 488 and PI Dead Cell Apoptosis Kit with Annexin V FITC and PI Annexin V-FITC and PI apoptosis kit Alexa Fluor® 488 annexin V/Dead Cell Apoptosis Kit with Alexa® Fluor 488 annexin V and PI Alexa Fluor 488 annexin V/Dead Cell Apoptosis Kit with Alexa Fluor 488 annexin V and PI for Flow Cytometry FITC Annexin V/Dead Cell Apoptosis Kit with FITC annexin V and PI Dead Cell Apoptosis Kit with annexin V-FITC and PI for flow cytometry Annexin V and PI

2 protocols using annexin 5 and pi apoptosis kit

Dendritic Cells Apoptosis Assay

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Apoptosis of dendritic cells was determined using an Annexin-V and PI apoptosis kit (BU-ap0102, Life Technologies, Carlsbad, CA, USA). After washing twice with PBS, cells were re-suspended in binding buffer and incubated with Annexin-V and PI for 15 min. At least 5000 cells were counted per sample. Apoptotic cells appeared as Annexin-V positive and PI negative.

Feng M., Li J., Wang J., Ma C., Jiao Y., Wang Y., Zhang J., Sun Q., Ju Y., Gao L, & Zhao Y. (2014). High glucose increases LPS-induced DC apoptosis through modulation of ERK1/2, AKT and Bax/Bcl-2. BMC Gastroenterology, 14, 98.

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Apoptosis Detection using Annexin-V and PI

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Apoptosis of neurons was identified using an Annexin-V and PI apoptosis kit (BU-ap0102, Life Technologies, Carlsbad, CA, USA). After washing twice with PBS, cells were resuspended in binding buffer and incubated with Annexin-V and PI for 15 min. At least 5000 cells were counted per sample. Apoptotic cells appeared as Annexin-V positive and PI negative [65 (link)].

Zhang J., Yao P., Han W., Luo Y., Li Y., Yang Y., Xia H., Chen Z., Chen Q., Wang H., Yang L., Li H., Hu C., Huang H., Peng Z., Tan X., Li M, & Yang J. (2022). Maternal Prenatal Inflammation Increases Brain Damage Susceptibility of Lipopolysaccharide in Adult Rat Offspring via COX-2/PGD-2/DPs Pathway Activation. International Journal of Molecular Sciences, 23(11), 6142.

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