Explants were pulverized in TRIzol (Catalog # 15596026; Life Technologies, Carlsbad, CA) under liquid nitrogen using a freezer mill (Model 6875; SPEX SamplePrep, Metuchen, NJ) for three cycles of 2 min each at maximum frequency with 2 min precooling between cycles. RNA was extracted by TRIzol/chloroform separation, according to the manufacturer’s protocol. RNA was then resuspended in lysis buffer and column purified, according to the Norgen RNA extraction kit protocol (Catalog # 48300; Norgen Biotek Corp).
Model 6875
Manufactured by SPEX
The SPEX Model 6875 is a laboratory equipment designed for sample preparation. It is a high-speed centrifuge capable of separating solid and liquid components within a sample. The equipment operates at variable speeds to accommodate different sample volumes and compositions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using model 6875
1
Efficient RNA Extraction from Plant Tissue
2
RNA Extraction from Plant Tissue
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