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Criterion xt 12 bis tris precast gels

Manufactured by Bio-Rad
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The Criterion XT 12% Bis-Tris precast gels are a laboratory equipment product manufactured by Bio-Rad. The gels are designed for protein separation and analysis using electrophoresis techniques.

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Lab products found in correlation

Semi dry electrotransfer system, by Bio-Rad (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Chemidoc digital imager, by Bio-Rad (1 mentions)

Close spelling variants of this product

Precast gels (207 citations) Bis-Tris gels (123 citations) Criterion gels (84 citations) Criterion XT Bis-Tris gels (76 citations) Criterion precast gels (71 citations) Criterion XT (33 citations) Criterion™ XT Bis-Tris Precast Gels (30 citations) Criterion XT Precast Gel (25 citations) Bis-Tris precast gels (13 citations) 12% Bis-Tris Criterion gels (8 citations)

2 protocols using criterion xt 12 bis tris precast gels

1

Western Blot Analysis of PrP

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PMCA samples were run on Criterion XT 12% Bis-Tris precast gels (Biorad, Hercules, CA, USA), electrotransferred onto nitrocellulose membranes with the semi-dry electrotransfer system (Biorad) and probed with biotinylated Sha31 anti-PrP monoclonal antibody62 (link), as described above. PrPC content of the cell lysates was determined by western blotting with SAF3462 (link) anti octarepeat region of PrP. Quantification was determined with the GeneTools software after acquisition of the signals with a GeneGnome digital imager.
Moudjou M., Chapuis J., Mekrouti M., Reine F., Herzog L., Sibille P., Laude H., Vilette D., Andréoletti O., Rezaei H., Dron M, & Béringue V. (2016). Glycoform-independent prion conversion by highly efficient, cell-based, protein misfolding cyclic amplification. Scientific Reports, 6, 29116.
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2

Western Blot for PrPres Detection

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PMCA samples were run on Criterion XT 12% Bis-Tris precast gels (Bio-Rad, Hercules, CA, USA), electrotransferred onto nitrocellulose membranes (Bio-Rad) and probed with biotinylated Sha31 anti-PrP monoclonal antibody45 (link). Streptavidin-peroxidase incubation and ECL detection were achieved as described for the dot blot assay. When necessary, the PrPres content of PMCA samples was determined with ImageLab software after acquisition of the signals with a Chemidoc digital imager (Bio-Rad, USA).
Al-Dybiat I., Moudjou M., Martin D., Reine F., Herzog L., Truchet S., Berthon P., Laude H., Rezaei H., Andréoletti O., Béringue V, & Sibille P. (2019). Prion strain-dependent tropism is maintained between spleen and granuloma and relies on lymphofollicular structures. Scientific Reports, 9, 14656.
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