Total RNA of pooled larvae was extracted using TRIZol reagent (Invitrogen, Life Technologies) according to the manufacturer’s instructions with DNase I treatment to remove genomic DNA contamination. Quality of RNA was assessed with an Agilent 2100 Bioanalyzer and concentration was measured on a NanoDrop ND-2000 Spectrophotometer. Library preparation of poly(A)-enriched RNA (NEBNext® Ultra™ II Directional RNA Library) and RNA-Seq were done by CES Génome Québec (Montréal, Québec, Canada) on an Illumina NovaSeq 6000 platform. Four samples were discarded because of poor RNA quality: One replicate from the conventional condition for each time point and one replicate from the axenic condition for day 12. Therefore, all following statistical tests are done with 5 replicates by condition by time.
Nebnext ultra 2 directional rna library
Manufactured by Illumina
Sourced in Canada
The NEBNext® Ultra™ II Directional RNA Library Prep Kit is a laboratory equipment product designed for the preparation of directional RNA sequencing libraries. The kit provides a streamlined workflow for converting RNA into adapter-ligated DNA fragments, which can then be used for next-generation sequencing applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nebnext ultra 2 directional rna library
1
Transcriptome Analysis of Larval Samples
2
RNA Sequencing of Larval Samples
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Total RNA of pooled larvae was extracted using TRIZol reagent (Invitrogen, Life Technologies) according to the manufacturer's instructions with DNase I treatment to remove genomic DNA contamination. Quality of RNA was assessed with an Agilent 2100 Bioanalyzer and concentration was measured on a NanoDrop ND-2000 Spectrophotometer. Library preparation of poly(A)-enriched RNA (NEBNext® Ultra™ II Directional RNA Library) and RNA-Seq were done by CES Génome Québec (Montréal, Québec, Canada) on an Illumina NovaSeq 6000 platform. Four samples were discarded because of poor RNA quality: One replicate from the conventional condition for each time point and one replicate from the axenic condition for day 12. Therefore, all following statistical tests are done with 5 replicates by condition by time.
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