Specific antibody against phospho fyn

DMEM, MEM-α medium, 1 × DPBS, antibiotics, and fetal bovine serum (FBS) were purchased from GE Healthcare Life Sciences (Hyclone™, Logan, UT, USA). The EZ-Cytox cell viability assay kit was obtained from Daeil Lab Service Co., Ltd. (Seoul, Korea). Specific antibodies against phospho-Akt, phospho-cPLA₂, phospho-ERK, phospho-JNK, phospho-Lyn, phospho-p38, phospho-PKCδ, phospho-PLCγ1/2, phospho-Syk, and COX-2 were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). A specific antibody against phospho-Fyn was obtained from Biorbyt Ltd. (Cambridge, UK). A specific antibody against β-actin was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). A specific antibody against phospho-5-LO and EIA kits for PGD₂ and LTC₄ were obtained from Cayman Chemical Co. (Ann Arbor, MI, USA). ELISA kits for IL-4 and TNF-α were purchased from e-Bioscience, Inc. (Science Center Drive, San Diego, CA, USA). A specific antibody against MMP-1 was obtained from R&D Systems, Inc. (Minneapolis, MN, USA). Specific antibodies against MMP-3 and MMP-13 were purchased from Abcam, Inc. (Cambridge, UK). 4-Nitrophenyl N-acetyl-β-d-glucosaminide (p-NAG), casein, DNP-HSA, DNP-IgE, trichloroacetic acid, caffeic acid, diethylene glycol, Folin-Ciocalteu reagent, and quercetin were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). All other chemicals were of analytical grade.

MEM-α medium, 1 × DPBS, penicillin, streptomycin and fetal bovine serum (FBS) were purchased from GE Healthcare Life Sciences (Hyclone™, Logan, UT, USA). The EZ-Cytox cell viability assay kit was obtained from Daeillab Service Co. (Seoul, Korea). Specific antibodies against phospho-cPLA₂, phospho-ERK, phospho-JNK, phospho-LYN, phospho-p38, phospho-PKCδ, phospho-PLCγ1, phospho-SYK and COX-2 were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). A specific antibody against phospho-FYN was obtained from Biorbyt Ltd. (Cambridge, UK). A specific antibody against α-tubulin was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). An enzyme immunoassay (EIA) kit for PGD₂ was obtained from Cayman Chemical Co. (Ann Arbor, MI, USA). Enzyme-linked immunosorbent assay (ELISA) kits for TNF-α, IL-4, and IL-6 were purchased from e-Bioscience, Inc. (San Diego, CA, USA). 4-Nitrophenyl-N-acetyl-β-d-glucosaminide (p-NAG), dinitrophenyl-human serum albumin (DNP-HSA) and DNP-immunoglobulin E (DNP-IgE) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). All other chemicals were of analytical grade.