Recombinant s1 and rbd proteins

Manufactured by BioLegend

Recombinant S1 and RBD proteins are laboratory-produced proteins derived from the SARS-CoV-2 virus. The S1 protein represents the spike protein's S1 subunit, while the RBD protein is the receptor-binding domain of the spike protein. These proteins can be used in various research and diagnostic applications.

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Lab products found in correlation

Bv421 conjugated streptavidin, by BioLegend (1 mentions)

2 protocols using recombinant s1 and rbd proteins

Preparation of B Cell-Specific Tetramers

Cited 1 time

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B cell-specific tetramers were prepared as described^{55 (link)}. A biotinylated form of recombinant S1 and RBD proteins (BioLegend) was tetramerized by the addition of PE-conjugated or BV421-conjugated streptavidin (BioLegend) and used for B cell tetramer staining assays. Briefly, SA-PE or SA-BV21 was added in an amount equal to 1/5 of the monomer substrate amount. SA was added in 5 equal portions to the monomer and incubated each time at 4 °C for 20 min on a shaker. Protease inhibitor was added to the tetramers at a 1x final concentration (Sigma). The tetramers were filled up to 100 µl with 0.1% BSA in PBS and stored at 4 °C.

Jacobsen E.M., Fabricius D., Class M., Topfstedt F., Lorenzetti R., Janowska I., Schmidt F., Staniek J., Zernickel M., Stamminger T., Dietz A.N., Zellmer A., Hecht M., Rauch P., Blum C., Ludwig C., Jahrsdörfer B., Schrezenmeier H., Heeg M., Mayer B., Seidel A., Groß R., Münch J., Kirchhoff F., Bode S.F., Strauss G., Renk H., Elling R., Stich M., Voll R.E., Tönshof B., Franz A.R., Henneke P., Debatin K.M., Rizzi M, & Janda A. (2022). High antibody levels and reduced cellular response in children up to one year after SARS-CoV-2 infection. Nature Communications, 13, 7315.

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SARS-CoV-2 S1 and RBD Tetramer Staining

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A biotinylated form of recombinant S1 and RBD proteins (BioLegend) were tetramerized by addition of PE-conjugated or BV421-conjugated streptavidin (BioLegend) and used for B cell tetramer staining assays. In brief, streptavidin-PE or streptavidin-BV21 was added in an amount that equals one-fifth of the monomer substrate amount. The streptavidin was added in five equal portions to the monomer and incubated each time at 4 °C for 20 min on a shaker. The tetramers were filled up to 100 μl with 0.1% BSA in PBS and stored at 4 °C.

Oberhardt V., Luxenburger H., Kemming J., Schulien I., Ciminski K., Giese S., Csernalabics B., Lang-Meli J., Janowska I., Staniek J., Wild K., Basho K., Marinescu M.S., Fuchs J., Topfstedt F., Janda A., Sogukpinar O., Hilger H., Stete K., Emmerich F., Bengsch B., Waller C.F., Rieg S., S.a.g.a.r., Boettler T., Zoldan K., Kochs G., Schwemmle M., Rizzi M., Thimme R., Neumann-Haefelin C, & Hofmann M. (2021). Rapid and stable mobilization of CD8+ T cells by SARS-CoV-2 mRNA vaccine. Nature, 597(7875), 268-273.

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