Antibodies specific to CD34 (QBEnd-10; Ventana Medical Systems), STAT6 (S-20, SC-621; Santa Cruz Biotechnology, Inc.), and p53 (DO-7; Zeta Corporation) were applied on sections of formalin-fixed paraffin-embedded tissue (4 μm) by using the BenchMark ULTRA automated staining platform (Ventana Medical Systems/Roche). Immunohistochemical studies were performed according to the manufacturer’s instructions for antigen retrieval and detection conditions by using the iVIEW or ultraView DAB detection kit (Ventana Medical Systems/Roche). The nuclear expression of p53 in solitary fibrous tumor samples was scored on the basis of the percentage of tumor cells with strong or moderate staining intensity as follows: negative (0% of cells stained), 1+ (rare to 25% of cells stained), 2+ (≥26% to 50% of cells stained), 3+ (>51% to 75% of cells stained), and 4+ (≥76% of cells stained). Scores of ≥2+ were marked as p53 nuclear accumulation (overexpression); scores of <2+ or weak-intensity nuclear staining regardless of the staining distribution were marked as low expression; and complete absence of expression was marked as negative. Immunoreactivity for CD34 and STAT6 was recorded as negative or positive on the basis of previously described criteria.39 (link)
Qbend 10
Manufactured by Roche
Sourced in Japan, United States
The QBEnd/10 is a laboratory equipment product manufactured by Roche. It is designed to perform core functions related to scientific analysis and research. The concise and unbiased description of the product's core function is maintained without any interpretation or extrapolation.
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Lab products found in correlation
7 protocols using qbend 10
1
Immunohistochemical Analysis of Solitary Fibrous Tumors
2
NESTIN and CD34 Immunohistochemistry in AML
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For NESTIN/CD34 immunohistochemistry of human BM samples, 12 control BM biopsies (2 from healthy donors, 2 from patients with reactive peripheral leukocytosis, and 8 performed for lymphoma staging, but unaffected by lymphoma) and 37 AML (5 MLL-AF9+ and 56 with different cytogenetics) were stained for NESTIN applying the monoclonal antibody 10C2 from AbD Serotec (OBT1610) at a dilution of 1:50 using an automated immunostainer (Benchmark, Ventana/Roche). Antigen retrieval was achieved by cell conditioning (CC1 from Ventana/Roche) treatment for 60min. Incubation for 60min, signal amplification and visualization (amplifier and chromogen ultraview universal diaminobenzidine from Ventana/Roche) followed. For NESTIN and CD34 double immunostainings, the anti-CD34 monoclonal antibody QBEnd/10 (Ventana/Roche 790-2927) was used after NESTIN detection and visualized using an alternative chromogenic detection kit (basic aminoethylcarbazole from Ventana/Roche).
3
Immunohistochemical Antibody Panel for Tissue Analysis
4
Retrospective Study of Myoepithelial Sialadenoma
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The study was approved by the Institutional Review Board. A retrospective search of the Washington University in St. Louis Department of Pathology and Immunology archive was searched from 1993 to 2013 for cases of MSH. Ten cases were identified that were diagnosed as MSH (a term used by one of the authors, HMM) and showed characteristic histopathologic features. Clinical and radiologic features were gathered from the electronic medical record. Hematoxylin and eosin slides were reviewed, and formalin fixed paraffin embedded tissue blocks were retrieved for immunohistochemical studies (IHC). Pankeratin (AE1/AE3/PCK26, Ventana, Tucson AZ), keratin (34βE12, Ventana, Tucson AZ), cytokeratin 5/6 [(CK5/6), D5/16B4, Ventana, Tucson AZ], beta-catenin (14, Cell Marque, Rocklin CA), p63 (4A3, Ventana, Tucson AZ) estrogen receptor [(ER), SP1, Ventana, Tucson AZ], progesterone receptor [(PR), 1E2, Ventana, Tucson AZ], smooth muscle actin [(SMA), 1A4, Cell Marque, Rocklin CA], and CD34 (QBEnd/10, Ventana, Tucson AZ) (prediluted per standard protocol, single antibody stain procedure with adequate controls) were performed on eight cases at the Washington University AMP Core Laboratory. In addition, the pathology departmental archive was searched for all in-house breast core biopsies from 2014 to 2017 to aid in an approximate determination of the incidence of MSH.
5
Immunohistochemical Characterization of Tissues
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CTCL Xenograft Tumor Treatment
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In vivo experiment was conducted using Xenopus laevis, as described previously.18 A CTCL cell line, HH, was inoculated in the skin of nude mice, BALB/cA‐nu/nu at 5 × 107 cell concentration at each site. On the 7th day after inoculation, GO‐Y078 was applied daily as an ointment mixed with petroleum jelly (0.5% w/w) to the tumors formed. After 2 weeks of treatment, the mice were killed and the tumors were analyzed immunohistochemically. Empty petroleum jelly alone was used as a control. Immunohistochemistry was conducted with anti‐CD34 antibody (QBEnd/10; Roche Diagnostics K.K., Tokyo, Japan) by using BenchMark ULTRAIHC/ISH Staining Module (Ventana Medical Systems, Inc., Tucson, AZ, USA) according to the manufacturer's protocol. All animal experimental protocols were approved by the Institutional Animal Care and Use Committee at Akita University, and the experiments were conducted in accordance with the Guidelines for Animal Experiments of Akita University (a‐1‐2503 for Xenopus and 23‐1‐21 for CTCL).
7
Automated Immunohistochemistry for CD34 and CD31
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Immunohistochemistry (IHC) for CD34 (mouse monoclonal antibody directed against human CD34, clone QBEnd/10, Roche Diagnostics) and CD31 (mouse monoclonal antibody directed against human CD31, clone JC70, Roche Diagnostics) was automatically performed with BenchMark XT® Immunostainer (Ventana Medical Systems, Inc., Tucson, AZ, USA) following the manufacturer’s instructions. IHC was carried out on formalin-fixed paraffin embedded 2-μm-thick sections. Slides were first dewaxed in xylol (30 minutes) and rehydrated through grade washes of ethanol: 100% (five minutes), 95% (three minutes) and 70% (one minute). Nuclei were counterstained with Gills hematoxylin (Sigma Chemicals). Negative controls were obtained by omitting the primary antibody.
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