3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt rpmi 1640

HPLC methanol, acetonitrile, trifluoroacetic acid (TFA), formic acid, and ultrapure water (Milli-Q Millipore) were used throughout the study. Metronidazole (total impurities: ≤0.0005% phosphorus and ≤0.1% insoluble matter), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), RPMI-1640 and Dulbecco's Modified Eagle’s Medium (DMEM) were obtained from Sigma-Aldrich, St. Louis, MO, USA. Sephadex® LH20 was obtained from GE Healthcare Life Sciences. The matrix 2,5-dihydroxybenzoic acid (DHB) was purchased from Bruker Daltonics, and NaCl was obtained from Synth.

Acrylamide and Curcumin (Sigma-Aldrich), mouse monoclonal IgG anti-p53 (DO-1) antibody, goat polyclonal IgG anti-cleaved Caspase 3 (P11) antibody, mouse monoclonal IgG anti β actin (2A3) antibody, goat anti-mouse IgG-HRP (sc-2031), mouse anti-rabbit IgG-HRP (sc-2357) and mouse anti-goat IgG-HRP: sc-2354 were from Santa Cruz Biotechnology (Dallas, TX, USA). Rabbit polyclonal IgG anti-Cytochrome P450 2E1 (ab28146) antibody was from Abcam, Cambridge, MA, USA. These antibodies were documented to be validated for specificity by the suppliers. SYBR Green PCR Master Mix from Bio-Rad (Austria). Ethidium bromide, acridine orange, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), RPMI-1640, and fetal bovine albumin (FBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethylenediaminetetraacetic acid (EDTA), phosphate buffered saline (PBS), were purchased from Sigma-Aldrich (St. Louis, MO, USA). HEPES were purchased from from Thermo Fisher Scientific (Waltham, MA, USA).

ATQ was purchased from Hubei Vanz Pharm, China (Ca No. 95233–18-4). The exosome isolation kit was provided by Cib Biotech Co. (Tehran, Iran). 3-(4,5-Dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide (MTT), RPMI-1640, fetal bovine serum (FBS), and penicillin/streptomycin were obtained from Sigma-Aldrich, USA. The bicinchoninic acid (BCA) protein quantification kit was purchased from DNA Biotech Co (Tehran, Iran). Dimethyl sulfoxide (DMSO) and ethanol were supplied by Merck, Germany.