The Muse Cell Analyzer was used to perform annexin-V and caspase 3/7 activity assays. Early/late apoptotic cell death or necrosis was detected using the Annexin-V/Dead Cell Kit (MCH100105, Millipore). The Caspase-3/7 Kit was chosen to investigate the activity of caspase 3 and 7, which are activated in apoptotic cell death (MCH100108, Millipore).To perform the assays, MDA-MB-231 cells were seeded at a density of 2 × 105 cells per well of 6-well plates. A 12.5 µM concentration of the compound was applied 24 h later, and the cells were incubated for an additional 12 and 24 h. At the end of the treatment period, the cells were trypsinized and analyzed for the detection of annexin-V stained cells and caspase 3/7 activity according to the manufacturer’s protocol (Millipore, Hayward, CA, USA).
Caspase 3 7 kit
Manufactured by Merck Group
Sourced in United States
The Caspase-3/7 Kit is a laboratory tool used to measure the activity of the caspase-3 and caspase-7 enzymes. Caspases are a family of enzymes that play a crucial role in the process of apoptosis, or programmed cell death. The kit provides a standardized and reliable method for quantifying the levels of these enzymes in various biological samples.
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Lab products found in correlation
2 protocols using caspase 3 7 kit
1
Apoptosis and Caspase Assays in MDA-MB-231 Cells
2
Cytotoxicity of Cisplatin and PEGylated Cisplatin
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Analysis of cell death was carried out with cultures of A549 (ATCC® CCL-185™) cell line, incubated for 48 and 72 hours in the absence or in the presence of increasing concentrations of cisplatin and PEGylated cisplatin (50 and 80 μM; or 39 and 62 μM (Pt)). Apoptotic cells were analyzed using the Muse™ Count, Viability kit, Muse™ Caspase 3/7 kit and the Muse™ Annexin V and Dead Cell Assay kit (Muse™ Cell Analyzer; Millipore, Billerica, MA, USA). Cell cycle analysis was performed by using the Muse™ Cell Cycle Kit according to manufacturer's instructions at two concentrations of Cisplatin and CDDPPEG. We obtained the percentage of viable, dead, apoptotic, G1, S and G2/M cells.
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