Hybond p pvdf transfer membrane

Manufactured by Merck Group

Hybond-P PVDF transfer membrane is a polyvinylidene difluoride (PVDF) membrane designed for protein transfer and detection in western blotting applications. It provides a stable and efficient platform for the transfer of proteins from polyacrylamide gels to a solid support for further analysis and detection.

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Lab products found in correlation

Irdye 800cw goat anti rabbit antibody, by LI COR (3 mentions) Irdye 680cw goat anti mouse antibody, by LI COR (3 mentions) Bca protein assay kit, by Cole-Parmer (3 mentions) Mouse anti gapdh, by Abcam (3 mentions) Rabbit anti lamin b1, by Abcam (2 mentions) Un scan it gel version 6, by Silk Scientific (2 mentions) Rabbit anti β actin, by Abcam (1 mentions) Irdye 800cw goat anti mouse antibody, by LI COR (1 mentions) Mouse anti glutamine synthetase, by Abcam (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Un scan it gel version, by Silk Scientific (1 mentions)

Close spelling variants of this product

PVDF transfer membrane Hybond-P membrane Hybond-P Transfer membranes PVDF membranes PES membrane

3 protocols using hybond p pvdf transfer membrane

Quantification of Hippocampal Proteins

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Protein concentration in hippocampal extracts from mice and human tissue was
measured using the BCA Protein Assay Kit (Cole-Parmer). Forty micrograms of
protein/lane were electrophoretically separated on a 12% SDS polyacrylamide gel
and electrically transferred onto a Hybond-P PVDF transfer membrane (Millipore)
for 1 h. Membranes were blocked in PBS-milk 5% at RT for 1 h. Next, membranes
were incubated in block solution overnight with the following primary
antibodies: mouse anti-glutamine synthetase (1:500; Abcam), mouse anti-GAPDH
(1:1,000; Abcam), rabbit anti-Cyclophilin B (1:1,000; Sigma) and rabbit
anti-β-actin (1:1,000; Abcam). Membranes were incubated for 1 h with IRDye 680CW
goat anti-mouse antibody, IRDye 800CW goat anti-mouse antibody, or IRDye 800CW
goat anti-rabbit antibody (LI-COR, 1:20,000), then scanned with an Odyssey
infrared imaging system (LI-COR) and analyzed using Un-Scan-It gel version 6.1
(Silk Scientific).

Matias I., Diniz L.P., Araujo A.P., Damico I.V., de Moura P., Cabral-Miranda F., Diniz F., Parmeggiani B., de Mello Coelho V., Leite R.E., Suemoto C.K., Ferreira G.C., Kubrusly R.C, & Gomes F.C. (2023). Age-Associated Upregulation of Glutamate Transporters and Glutamine Synthetase in Senescent Astrocytes In Vitro and in the Mouse and Human Hippocampus. ASN NEURO, 15, 17590914231157974.

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Western Blot Protein Quantification

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Protein concentration in cell extracts was measured by the BCA Protein Assay Kit (Cole‐Parmer). Forty micrograms protein/lane was electrophoretically separated on a 10% SDS polyacrylamide gel and electrically transferred onto a Hybond‐P PVDF transfer membrane (Millipore) for 1.5 h. Membranes were blocked in PBS‐milk 5% at RT for 1 h. Next, membranes were incubated in block solution overnight with the following primary antibodies: rabbit anti‐lamin‐B1 (1:1000; Abcam) and mouse anti‐GAPDH (1:1000; Abcam). Membranes were incubated for 1 h with IRDye 680CW goat anti‐mouse antibody and IRDye 800CW goat anti‐rabbit antibody (LI‐COR, 1:20,000) and then scanned and analyzed using Un‐Scan‐It gel version 6.1 (Silk Scientific).

Matias I., Diniz L.P., Damico I.V., Araujo A.P., Neves L.D., Vargas G., Leite R.E., Suemoto C.K., Nitrini R., Jacob‐Filho W., Grinberg L.T., Hol E.M., Middeldorp J, & Gomes F.C. (2021). Loss of lamin‐B1 and defective nuclear morphology are hallmarks of astrocyte senescence in vitro and in the aging human hippocampus. Aging Cell, 21(1), e13521.

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Western Blot Protein Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein concentration in cell extracts was measured by the BCA Protein Assay Kit (Cole-Parmer). Forty micrograms protein/lane was electrophoretically separated on a 10% SDS polyacrylamide gel and electrically transferred onto a Hybond-P PVDF transfer membrane (Millipore) for 1.5 h. Membranes were blocked in PBS-milk 5% at RT for 1 h. Next, membranes were incubated in block solution overnight with the following primary antibodies: rabbit anti-lamin-B1 (1:1,000; Abcam) and mouse anti-GAPDH (1:1,000; Abcam). Membranes were incubated for 1 h with IRDye 680CW goat antimouse antibody and IRDye 800CW goat anti-rabbit antibody (LI-COR, 1:20,000) and then scanned and analyzed using Un-Scan-It gel version 6.1 (Silk Scientific).

Matias I., Diniz L.P., Damico I.V., Neves L.d.S., Araújo A.P.B., Vargas G., Leite R.d.F., Suemoto C.K., Nitríni R., Filho W.J., Grinberg L.T., Hol E.M., Middeldorp J., & Gomes F.C.A. (2021). Loss of lamin-B1 and defective nuclear morphology are hallmarks of astrocyte senescencein vitroand in the aging human hippocampus.

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