Mini-PROTEAN Tetra electrophoresis and blots, a protein semi-dry transfer device, and a gel imaging system were used (Bio-Rad, Hercules, USA). A full wavelength microplate reader (Tecan, Zurich, Switzerland), an automatic high-speed refrigerated centrifuge (Sigma-Aldrich), a SCIENTZ-48 Tissue Grinder (Xinzhi, Hunan, China), an X-Ray Micro-CT SkyScan 1276 (Bruker, Billerica, USA), and a VS200 digital slide scanner (Olympus, Tokyo, Japan) were utilized for tissue analysis.
Vs200 digital slide scanner
Manufactured by Olympus
Sourced in United States, Japan
The Olympus VS200 is a digital slide scanner designed for high-resolution imaging of microscope slides. It captures digital images of specimen slides with a maximum resolution of 0.25 micrometers per pixel.
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Lab products found in correlation
2 protocols using vs200 digital slide scanner
1
Protein Analysis Using Advanced Equipment
2
Histopathological Assessment of Tumor Tissues
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For histopathological examination, the tissues (tumor, heart, liver, spleen, lung, and kidney) were fixed in 10% normal fixative formalin solution, dehydrated by series of alcohol (30–100%) and xylene for 1.5 h, and embedded in wax at 60 °C. The sections were then stained with hematoxylin and eosin (H&E). To observe the immune infiltration and anti‐inflammatory levels of tumor tissues, CD68 and CD206 immunohistochemistry (IHC) was performed according to the manufacturer's specifications (Yeasen Biotechnology (shanghai) Co.,Ltd., China). The positive cells were stained in brownish yellow and nuclei were counterstained in blue with hematoxylin. The sections were scanned by the VS200 digital slide scanner (Olympus, Japan) for observation of the staining results. The positive rate was quantified and analyzed with Image J software (NIH).
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