Anti c myc a19032

The following antibodies were purchased: anti-BRD4 (E2A7X) from Cell Signaling Technology, anti-BRD2 (D89B4) from Cell Signaling Technology, anti-BRD3 (2088C30) from Abcam Technology, anti-c-Myc (A19032) from ABclonal Technology, anti-c-Met (25869-1-AP) from Proteintech Technology, anti-EGFR (2232) from Cell Signaling Technology, and anti-GAPDH (AP0063) from Bioworld Technology Inc. All antibodies were utilized at 1:1000 dilutions, except GAPDH (1:10,000). JQ1 (HY-13030) and (2-Hydroxypropyl)-β-cyclodextrin (HY-101103) were purchased from Haoyuan Chemexpress Co., Ltd. Trichostatin A (T6270) was purchased from Target Mol, Inc. Vorinostat (T1583) was purchased from Target Mol, Inc. Osimertinib (HY-15772) was purchased from MedChemExpress (Monmouth Junction, NJ, USA). Reagents were dissolved in DMSO at 20 mmol/L, stored at -20℃, and diluted just before use. Lipofectamine 2000® transfection reagent (11668-019) was purchased from Life Technologies Co. Invitrogen (Carlsbad, CA, USA).

For chromatin immunoprecipitation (ChIP) assays, anti-histone H3, GTX122148 (GENETEX), anti-acetyl histone H3 (H3K27ac), #39134, 39336 (Active Motief), anti-Sp1, GTX110593 (GENETEX), A19649 (ABclonal), anti-c-Myc, A19032 (ABclonal), C15410174 (Diagenode), anti-c-MycK323ac, C15410346 (Diagenode) were used. The detailed procedures were provided in Supplementary Data S1 and Supplementary Table S1. qPCR analysis was performed using the Thermal Cycle Dice (Takara Bio). Several amplifications were performed by classic PCR and products were run on 1.5% agarose gels and was visualized on iBrightFL1000 (Thermo Fisher Scientific). Primer sequences were available in Supplementary Table S1.