Each compound was dissolved in MeCN and degassed using the freeze-pump-thaw method prior to measurement. Emission spectra were recorded at 25 °C using either a JASCO FP-8600 spectrofluorometer or a Hamamatsu C9920-02 system. The absolute emission quantum yields were evaluated using the Hamamatsu C9920-02 system equipped with an integrating sphere and a multichannel photodetector (PMA-12). Emission lifetimes were obtained using a HORIBA TemPro fluorescence lifetime system with an emission monochromator. The excitation light source was a NanoLED-560 pulse lamp and the instrumental response time was less than 0.1 ns. All emission decays were fitted by single or double exponential functions within appropriate χ 2 (0.96-1.24). Transient emission spectra were recorded by the point-to-point measurement method using a Horiba FluoroCube time-correlated single-photon counting system. Sensitivity of the system at each detection wavelength was calibrated using correction coefficients determined from the difference between [Ru(4,4′-dimethyl-2-2′bipyridine) 3 ] 2+ spectra recorded with the JASCO FP-8600 spectrofluorometer and the HORIBA TemPro fluorescence lifetime system.
Tempro fluorescence lifetime system
Manufactured by Horiba
Sourced in Japan
The TemPro fluorescence lifetime system is a laboratory instrument designed to measure the fluorescence lifetime of samples. It provides accurate and precise measurements of the time-resolved fluorescence properties of materials. The system features a pulsed excitation source, sample holder, and detection electronics to capture and analyze the fluorescence decay profile of the sample.
Automatically generated - may contain errors
2 protocols using tempro fluorescence lifetime system
1
Photophysical Characterization of Compounds
2
Spectroscopic Analysis of Phenothiazine Dyes and NADH
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The
absorption spectra of phenothiazine
dyes and NADH were measured with a UV–vis spectrophotometer
UV-1650PC (Shimadzu, Kyoto, Japan). The fluorescence spectra of the
samples were measured with an F-4500 fluorescence spectrophotometer
(Hitachi, Tokyo, Japan). The τf of phenothiazine
dyes was measured using a time-correlated single-photon counting method
with the TemPro Fluorescence Lifetime System (HORIBA, Kyoto, Japan).
Laser excitation at 637 nm was achieved using a diode laser (NanoLED-635L,
HORIBA) at a repetition rate of 1.0 MHz. The experimental error of
this measurement is within 0.01 ns.
absorption spectra of phenothiazine
dyes and NADH were measured with a UV–vis spectrophotometer
UV-1650PC (Shimadzu, Kyoto, Japan). The fluorescence spectra of the
samples were measured with an F-4500 fluorescence spectrophotometer
(Hitachi, Tokyo, Japan). The τf of phenothiazine
dyes was measured using a time-correlated single-photon counting method
with the TemPro Fluorescence Lifetime System (HORIBA, Kyoto, Japan).
Laser excitation at 637 nm was achieved using a diode laser (NanoLED-635L,
HORIBA) at a repetition rate of 1.0 MHz. The experimental error of
this measurement is within 0.01 ns.
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