Insulin transferrin selenium g

Manufactured by Thermo Fisher Scientific

Sourced in United States

Insulin-Transferrin-Selenium-G is a cell culture supplement used to support cell growth and proliferation in vitro. It provides a combination of insulin, transferrin, and selenium to promote cell survival and development.

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Lab products found in correlation

Dexamethasone (dex), by Merck Group (1 mentions) Hepg2 human liver cells, by American Type Culture Collection (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Penicillin g, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Fibronectin, by Merck Group (1 mentions) Brdu elisa kit, by Roche (1 mentions) Rhil 4, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Insulin (904 citations) Insulin-transferrin-selenium (622 citations) Transferrin (151 citations) Selenium (75 citations) Insulin-transferrin-selenium (ITS-G, (19 citations) Insulin-transferrin-selenium-G supplement (11 citations)

4 protocols using insulin transferrin selenium g

Culturing HepG2 and AML12 Liver Cells

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The HepG2 human liver cells (ATCC, Manassas, VA, USA) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Hyclone, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone), 4 mM glutamine (Hyclone), 100 U/mL penicillin (Hyclone), and 100 μg/mL streptomycin (Hyclone). The AML12 mouse liver cells (ATCC) were grown in DMEM/F-12 medium (Hyclone) supplemented with 10% FBS, 2.5 mM glutamine, 15 mM HEPES, 100 U/mL penicillin, and 100 μg/mL streptomycin. The medium was supplemented with 1X Insulin-Transferrin-Selenium-G (Gibco, Life Technologies™, New York, NY, USA), and 40 ng/mL dexamethasone (Sigma-Aldrich, St. Louis, MO, USA). The cells were incubated at 37 °C in a humidified atmosphere and 5% CO₂.

Kim S.H., Kwon D., Lee S., Ki S.H., Jeong H.G., Hong J.T., Lee Y.H, & Jung Y.S. (2019). Polyhexamethyleneguanidine Phosphate-Induced Cytotoxicity in Liver Cells Is Alleviated by Tauroursodeoxycholic Acid (TUDCA) via a Reduction in Endoplasmic Reticulum Stress. Cells, 8(9), 1023.

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Cell Culture Conditions for Various Cell Lines

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African green monkey kidney (Vero, KCLB 10081) cells were maintained in Dulbecco’s Modified Eagles Medium (DMEM, Hyclone, Logan, UT, USA) complemented with 10% (v/v) fetal bovine serum (FBS, Hyclone), 100 U/mL penicillin G (Hyclone), and 100 μg/mL streptomycin (Hyclone). Intestinal porcine epithelial cells from jejunum (IPEC-J2) cells were kindly obtained from Dr. Hyun Jang (Libentech Co., Ltd., Daejeon, Republic of Korea) and maintained in DMEM supplemented with 10% FBS, 1X Insulin-Transferrin-Selenium-G (Gibco, Billings, MT, USA), 100 U/mL penicillin G, and 100 μg/mL streptomycin. Porcine alveolar macrophage 3D4/21 (ATCC CRL-2843™) cells were maintained in the Roswell Park Memorial Institute 1640 medium (RPMI-1640, Gibco), complemented with 10% FBS, 100 U/mL penicillin G, and 100 μg/mL streptomycin. The cells were maintained at 37 °C under 5% CO₂.

Park J.E, & Shin H.J. (2022). Resistance of Field-Isolated Porcine Epidemic Diarrhea Virus to Interferon and Neutralizing Antibody. Veterinary Sciences, 9(12), 690.

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Neuronal Differentiation of hESCs and hiPSCs

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Neuronal differentiation was performed as described previously with slight modifications^{51 (link)}. Briefly, hESCs were dissociated and plated on bacterial dishes in hESC medium without bFGF for 1 week to allow formation of embryoid bodies (EB). EBs were allowed to attach to tissue culture dish and neuronal precursors were selected by incubation in DMEM/F-12 medium supplemented with 2mM L-glutamine, Insulin-Transferrin-Selenium-G (Invitrogen), and fibronectin (Sigma-Aldrich) for 30 days. hESCs and hiPSCs in vitro spontaneous differentiations were performed by culturing in serum-free ITSFn medium for different periods of time up to 12 days without EB formation.

Cha Y., Han M.J., Cha H.J., Zoldan J., Burkart A., Jung J.H., Jang Y., Kim C.H., Jeong H.C., Kim B.G., Langer R., Kahn C.R., Guarente L, & Kim K.S. (2017). Metabolic control of primed human pluripotent stem cell fate and function by the miR-200c–SIRT2 axis. Nature cell biology, 19(5), 445-456.

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Cell Proliferation Inhibition Assay

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Inhibition of cell proliferation was determined using the colorimetric cell proliferation ELISA BrdU kit from Roche Applied Science (Indianapolis, IN) as described before^{68 (link)}. Briefly, cells were cultured in 96 wells plates (100 µL/well) at a cell density of 2×10⁵ cells/mL in IMDM medium supplemented with 10% FBS, 100 U/mL penicillin, streptomycin 100 µg/mL, insulin-transferrin-selenium-G (all materials from Invitrogen, San Diego, CA) and 0.2 µg/mL of rhIL-4 (Peprotech, Rocky Hill, NJ). Cells were activated with 0.1 µg/mL of rhCD154 (MegaCD40L, Enzo Life Sciences) in the presence of various concentrations of test compounds. After 48 h, BrdU labeling solution was added as recommended, and cells were cultivated for another 48 h. Detection of incorporated BrdU was carried out following the instructions of the ELISA BrdU kit.

Chen J., Song Y., Bojadzic D., Tamayo-Garcia A., Landin A.M., Blomberg B.B, & Buchwald P. (2017). Small-Molecule Inhibitors of the CD40–CD40L Costimulatory Protein-Protein Interaction. Journal of medicinal chemistry, 60(21), 8906-8922.

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