Hrp conjugated goat anti mouse igg antibody ab214879

Immunohistochemical staining was performed as described previously [46] . Brie y, tumors removed from the xenografted mice were xed with 4% paraformaldehyde and the xed tissues were embedded in para n, sliced to a thickness of 4 µm, and placed on glass slides. After depara nization and rehydration, tissue antigens were retrieved with citrate buffer (pH 6), and endogenous peroxidase activity was inhibited by incubating the tissue sections with 3% H 2 O 2 for 10 min. The sections were then blocked with blocking solution (Blocking One Histo, Nacalai Tesque) and incubated with mouse anti-Ki67 antibody (M7240, dilution 1:100, DAKO, Glostrup, Denmark) for 20 min at room temperature. Incubation with the secondary antibody (HRP-conjugated goat anti-mouse IgG antibody; ab214879, pre-diluted, Abcam) was performed at room temperature for 30 min. Hematoxylin (8656, Sakura Finetek, Japan) was used for counterstaining the nucleus. The signals were detected by DAB (K3468, DAKO).