Polyclonal rabbit anti iba1

Manufactured by Abcam

Sourced in Denmark

Polyclonal rabbit anti‐Iba1 is an antibody that specifically binds to the Iba1 protein, which is a marker for macrophages and microglia. This antibody can be used for the detection and localization of Iba1 in various applications.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 488 donkey anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Polyclonal goat anti iba 1, by Abcam (1 mentions) Donkey antigoat igg h l alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Donkey antirabbit igg h l alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Donkey antimouse igg h l alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Donkey antigoat igg h l alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti neun, by Merck Group (1 mentions) Alexa fluor 594 donkey anti mouse igg h l, by Thermo Fisher Scientific (1 mentions) Brusatol, by Merck Group (1 mentions) 3 3 diaminobenzidine (dab), by Vector Laboratories (1 mentions) Extravidin peroxidase, by Merck Group (1 mentions) Biotinylated anti rabbit igg, by Vector Laboratories (1 mentions) Polyclonal rabbit anti gfap, by Agilent Technologies (1 mentions)

2 protocols using polyclonal rabbit anti iba1

Microglial Activation and Regulation Mechanisms

Check if the same lab product or an alternative is used in the 5 most similar protocols

The primary antibodies used in this study included polyclonal goat anti‐Iba1 (Abcam, 1:400), polyclonal rabbit anti‐Iba1 (Abcam, 1:400), polyclonal rabbit anti‐heme oxygenase‐1 (Enzo, 1:500), monoclonal mouse anti‐TLR9 (Abcam, 1:400), polyclonal rabbit anti‐TLR9 (GeneTex, 1:400), polyclonal goat anti‐GFAP (Abcam, 1:400), monoclonal rabbit anti‐Darpp‐32 (Cell Signaling, 1:100), polyclonal rabbit anti YM‐1 (Abcam, 1:200), monoclonal mouse anti‐NeuN (MERCK, 1:400), monoclonal mouse anti‐Arg1 (Santa Cruz, 1:200), polyclonal rabbit anti‐CD16 (Abcam, 1:200), polyclonal rabbit anti‐iNOS (Abcam, 1:200), polyclonal rabbit anti‐CD36 (GeenTex, 1:2000), monoclonal rabbit anti‐CD204 (Abcam, 1:2000), And polyclonal rabbit anti‐Nrf2 (Proteintech, 1:2000).
The secondary antibodies used in this article for immunofluorescence staining included donkey anti‐rabbit IgG (H + L) Alexa Fluor 488, donkey anti‐rabbit IgG (H + L) Alexa Fluor 594, donkey anti‐mouse IgG (H + L) Alexa Fluor 488, donkey anti‐mouse IgG (H + L) Alexa Fluor 594, donkey anti‐goat IgG (H + L) Alexa Fluor 488, and donkey anti‐goat IgG (H + L) Alexa Fluor 594, all obtained from Invitrogen.
The drugs used in this study included CpG ODN1826 and CpG ODN2138 (InvivoGen), clodronate liposomes, control liposomes (Liposoma Technology), and brusatol (Sigma‐Aldrich).

Wei J., Dai S., Pu C., Luo P., Yang Y., Jiang X., Li X., Lin W, & Fei Z. (2022). Protective role of TLR9‐induced macrophage/microglia phagocytosis after experimental intracerebral hemorrhage in mice. CNS Neuroscience & Therapeutics, 28(11), 1800-1813.

+ Open protocol

+ Expand

Immunohistochemical Detection of Astrocytes and Microglia

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serial, coronal frozen sections were immunostained for GFAP and Iba1 as described earlier.^{46 (link)} Sections were treated with 3% hydrogen peroxide to reduce the endogenous peroxidase activity. The sections were then incubated with polyclonal rabbit anti-GFAP (1:100, Dako, Denmark) or polyclonal rabbit anti-Iba-1 (1:500, Abcam, Cambridge, MA, USA) antibodies overnight at 4°C. The sections were incubated with biotinylated anti-rabbit IgG (1:20, Vector Labs, Burlingame, CA, USA) for 1 h, followed by incubation with ExtrAvidin peroxidase (1:20, Sigma Chemicals, St. Louis, USA) for another 1 h. The color was developed using DAB (Vector Labs, Burlingame, CA, USA) as chromogen. Sections were mounted on gelatin-coated slides, air dried, dehydrated in ethyl alcohol, cleared in xylene, and mounted with DPX. All the brain sections were examined and immunostained cells were counted by Olympus BX51 TF upright transmitted light microscope using 40× objective (aperture is 0.75).

Abd-El-Basset E.M., Rao M.S, & Alsaqobi A. (2020). Interferon-Gamma and Interleukin-1Beta Enhance the Secretion of Brain-Derived Neurotrophic Factor and Promotes the Survival of Cortical Neurons in Brain Injury. Neuroscience Insights, 15, 2633105520947081.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!