The delivery of insulin loaded on the MNs was studied, using abdominal porcine skin. The in vitro testing was carried out in Franz diffusion cells (PermeGear, Inc., Hellertown, PA, USA). Dermatomed skin samples were placed in phosphate buffered saline (PBS; pH 7.4) for 1 hour prior to experimentation. The MN arrays were inserted into the skin samples using manual finger pressure. The skin and MN array was mounted onto the donor compartment of a Franz diffusion cell. The temperature of the Franz cells was maintained at 37 °C using an automated water bath (Thermo Fisher Scientific, Newington, USA). Sample fractions (6–6.5 mL/h) were collected using an auto-sampler (FC 204 fraction collector, Gilson, Middleton, WI, USA) attached to the Franz diffusion cells. Statistical analysis for the drug release was performed by using a Mann–Whitney nonparametric test (InStat, GraphPad Software Inc., San Diego, CA, USA), where samples are considered to be statistically significant at p < 0.05.
Automated water bath
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Automated Water Bath is a laboratory equipment designed for precise temperature control and incubation. It maintains a consistent water temperature within a specified range, allowing for accurate temperature-dependent experiments and processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using automated water bath
1
In vitro Evaluation of Insulin-Loaded Microneedle Delivery
2
Microneedle-Mediated Insulin Release
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A total diffusion area of 1.1 cm 2 was used to assess the insulin release. The MN arrays were inserted into the abdominal porcine skin samples for 30 s, via manual finger pressure. The sample was then mounted onto the donor compartment of a Franz diffusion cell. The temperature of the Franz cells was maintained at 37ºC using an automated water bath (Thermo Fisher Scientific, Newington, USA). Sample fractions (6-6.5 mL h -1 ) were collected using an autosampler (FC 204 fraction collector, Gilson, USA) attached to the Franz diffusion cells system.
Statistical analysis for the drug release was performed by using a Mann-Whitney nonparametric test and t-test analysis for the in vivo studies (InStat, GraphPad Software Inc., San Diego, CA, USA), where samples were considered as statistically significant at p < 0.05.
Statistical analysis for the drug release was performed by using a Mann-Whitney nonparametric test and t-test analysis for the in vivo studies (InStat, GraphPad Software Inc., San Diego, CA, USA), where samples were considered as statistically significant at p < 0.05.
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