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Non esterified free fatty acid kit

Manufactured by Sekisui

The Non-esterified Free Fatty Acid Kit is a laboratory equipment used to quantitatively determine the concentration of non-esterified free fatty acids in biological samples. It provides a reliable and efficient method for measuring this important metabolic parameter.

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2 protocols using non esterified free fatty acid kit

1

Serum Biomarker Measurement in Mice

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Terminal bleeds from mice were incubated at room temperature for 1 hour in serum separator tubes (Sarstedt, Nümbrecht, Germany) and then spun at 10,000 × rpm for 5 minutes. Serum was collected and stored at −80°C until analysis. Mouse leptin, human NAG-1, mouse IGF-1 (R&D, Minneapolis, MN), mouse insulin (Alpco, Salem, NH), and mouse glycerol (Cayman, Detroit, MI) ELISA kits was used according to manufacturer’s instructions. Cholesterol and triglycerides kit was purchased from Beckman Coulter (Melvill, NY) and non-esterified free fatty acid kit was purchased from Sekisui Diagnostics (Exton, PA). Analysis was done using an Olympus AU400e clinical analyzer by Beckman Coulter.
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2

Serum Biomarker Measurement in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Terminal bleeds from mice were incubated at room temperature for 1 hour in serum separator tubes (Sarstedt, Nümbrecht, Germany) and then spun at 10,000 × rpm for 5 minutes. Serum was collected and stored at −80°C until analysis. Mouse leptin, human NAG-1, mouse IGF-1 (R&D, Minneapolis, MN), mouse insulin (Alpco, Salem, NH), and mouse glycerol (Cayman, Detroit, MI) ELISA kits was used according to manufacturer’s instructions. Cholesterol and triglycerides kit was purchased from Beckman Coulter (Melvill, NY) and non-esterified free fatty acid kit was purchased from Sekisui Diagnostics (Exton, PA). Analysis was done using an Olympus AU400e clinical analyzer by Beckman Coulter.
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