Crude membrane fractions (40 µg for spleen and 80 µg for liver) from mouse tissues were prepared and analyzed by western blotting as previously described (7 (link)). Antibodies were diluted in blocking solution as follows: anti-FPN (7 (link), 19 (link)): 1/200 (liver) or 1/500 (spleen), anti-HMOX1 (Stressgen): 1/4,000, anti-LAMP1 (DSHB): 1/500, and anti-TfR1 (Zymed): 1/200.
Anti tfr1
Manufactured by Zymo Research
Sourced in United States
Anti-TfR1 is a laboratory product designed to detect the transferrin receptor 1 (TfR1) protein. TfR1 is involved in the cellular uptake of iron and plays a role in iron homeostasis. The Anti-TfR1 product can be used to identify and quantify the expression of TfR1 in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Primary antibody dilution buffer, by Beyotime (2 mentions)
Nmdar2a, by Abcam (2 mentions)
Anti ferritin, by Abcam (2 mentions)
Nmdar1, by Abcam (2 mentions)
Rasd1, by Abcam (2 mentions)
Superoxide dismutase 2 sod2, by Abcam (2 mentions)
Mitoferrin1, by Abcam (2 mentions)
Anti irp2, by Alpha Diagnostic (2 mentions)
Anti dmt1, by Alpha Diagnostic (2 mentions)
Peroxidase conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions)
3 protocols using anti tfr1
1
Western Blot Analysis of Mouse Tissue
2
Protein Analysis in Hippocampus and Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Proteins were obtained from hippocampal tissue or cultured neurons or isolated mitochondria. For western blotting, 35-50 µg protein was added per lane of 12% SDS-PAGE. Primary antibodies were diluted in primary antibody dilution buffer (Beyotime Biotech., Shanghai, China). Antibodies used included: anti-GAPDH (Abgent Biotech. Co. Ltd., Suzhou, China), anti-ferritin, superoxide dismutase 2 (SOD2), Mitoferrin1, Drp1, Mfn2, RASD1, NMDAR1, and NMDAR2A (Abcam, Cambridge, MA, USA), anti-TfR1 (Zymed, San Francisco, CA, USA), anti-IRP2 (polyclonal, raised from rabbit), and anti-DMT1 (Alpha Diagnostic International, San Antonio, TX, USA). Detection was performed using peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, Waltham, UK). Quantification of the density of the western bands was done with programme ImageJ (http://rsb.info.nih.gov/ij/).
3
Quantitative Protein Analysis of Hippocampal Samples
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Proteins were obtained from the hippocampal tissue or cultured neurons or isolated mitochondria. For western blotting, 35–50 μg protein was added per lane of 12% SDS-PAGE. Primary antibodies were diluted in primary antibody dilution buffer (Beyotime Biotech., Shanghai, China). Antibodies used included the following: anti-GAPDH (Abgent Biotech. Co. Ltd., Suzhou, China), anti-ferritin, superoxide dismutase 2 (SOD2), Mitoferrin1, Drp1, Mfn2, RASD1, NMDAR1, and NMDAR2A (Abcam, Cambridge, MA, USA), anti-TfR1 (Zymed, San Francisco, CA, USA), anti-IRP2 (polyclonal, raised from rabbit), and anti-DMT1 (Alpha Diagnostic International, San Antonio, TX, USA). Detection was performed using peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, Waltham, UK). Quantification of the density of the western bands was done with programme ImageJ (http://rsb.info.nih.gov/ij/ ).
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