Keratinocytes and dermal fibroblasts were seeded at a density of 1 × 105 cells/well in a 24-well plate. Cells were then treated with gradient doses of KY19382 dissolved in DMSO for 24 h. Cell viability was assessed using the CellTiter-Glo mixture, as recommended by the supplier. Adenosine triphosphate (ATP) was quantified spectrophotometrically at 560 nm by a microplate luminometer (BMG Labtech).
Microplate luminometer
Manufactured by BMG Labtech
Sourced in Germany
The Microplate Luminometer is a lab equipment designed to measure the luminescence of samples in a microplate format. It provides accurate quantitative analysis of luminescent signals, supporting a wide range of applications in life science research and drug discovery.
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Lab products found in correlation
4 protocols using microplate luminometer
1
Evaluating Cell Viability with KY19382
2
Cell Viability Assay for Natural Compounds
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HaCaT keratinocytes and human dermal fibroblasts were plated at a density of 1 × 105 cells/well in a 24-well plate. The cells were then treated with DMSO, a gradient dose of E. daniellii extract, or hesperidin for 24 h. Cell viability was assessed using the CellTiter-Glo mixture, as recommended by the supplier. Adenosine triphosphate (ATP) was quantified spectrophotometrically at 560 nm using a microplate luminometer (BMG Labtech, Ortenberg, Germany).
3
Luciferase Assay for HEK293-TOP Cells
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HEK293-TOP cells were seeded at a density of 2.5 × 104 cells/well into a 96-well plate and incubated with DMEM containing 10% FBS for 24 h. Cells were incubated for 24 h with or without KY19382 dissolved in DMSO. All cell lysates were extracted with 25 μL of 1× reporter lysis buffer (Promega, Madison, WI, USA) per well, and luciferase activity was measured by a microplate luminometer (BMG Labtech, Offenburg, Germany).
4
Evaluating E. daniellii Extract Effects
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HEK293-TOP cells were seeded into 96-well plates at a density of 2.5 × 104 cells/well and were incubated in a medium containing 10% FBS for 24 h. The cells were incubated for 24 h with or without 1, 5, 10, 25, 50 μg/mL of E. daniellii extract or 1, 5, 10, 25, 50 μM of each of its components (Sigma-Aldrich). The total cell lysates were extracted with 25 μL of 1× reporter lysis buffer (Promega, Madison, WI, USA) per well, and luciferase activity was measured using a microplate luminometer (BMG Labtech, Offenburg, Germany).
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