Soybean lox

Butylated hydroxyanisole (BHA, ≥98.5%), chlorogenic acid (European Pharmacopoeia Reference Standard), diclofenac (≥98%), kojic acid (≥98.5%), (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, collagenase from Clostridium histolyticum, hyaluronidase from bovine testes, mushroom tyrosinase and porcine pancreas elastase were purchased from Sigma-Aldrich (St. Louis, MO, USA). Soybean LOX was a product from TCI chemicals (Tokyo, Japan). Acetonitrile was HPLC grade. Other reagents and chemicals were of analytical grade.

Butylated hydroxyanisole (BHA ≥ 98.5%), hyaluronidase from bovine testes, diclofenac (≥98%), nordihydroguaiaretic acid (NDGA ≥ 98.5%) kojic acid (≥98.5%), tannic acid, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, and mushroom tyrosinase were purchased from Sigma-Aldrich (St. Louis, MO, USA). Soybean LOX was a product from TCI chemicals (Tokyo, Japan). The spontaneously immortalized human keratinocyte line (HaCaT) was purchased from (CLS Cell Line Services, Heidelberg, Germany). The buffers and chemicals used for cell cultivation were as follows: Hank’s balanced salt solution (HBSS; pH 6.0) (Capricorn Scientific, Ebsdorfergrund, Germany), Dulbecco’s modified Eagle medium (DMEM) (Sigma-Aldrich, St. Louis, MO, USA), FBS (Biosera, Boussens, France), penicillin, streptomycin, and amphotericin B (5%, Lonza, Basel, Switzerland). The other reagents and chemicals were of analytical grade.

Root bark of Berberis vulgaris was a gift from Suban (Samobor, Croatia). Berberine chloride (≥98.5%), BHA (≥98.5%), kojic acid, diclofenac, α-glucosidase, α-amylase, LOX, and tyrosinase were purchased from Sigma-Aldrich (St.Louis, MO, USA), while soybean LOX was from purchased from TCI chemicals (Tokyo, Japan). Acetonitrile was of HPLC grade. Other reagents and chemicals were of analytical grade.