Mouse anti ha mab

The following antibodies were used in the present study: anti-tubulin mouse mAb (Covance, USA), anti-HA mouse mAb (Covance), anti-c-myc mouse mAb (Millipore, USA), anti-hexon goat pAb (Thermo Scientific, USA), anti-Ad5 pAb (Abcam, UK). The anti-Ad5 and anti-Ad37 rabbit polyclonal serum were a gift from Panyong Mao (Beijing 302 Hospital, Beijing, China). The anti-Ad5 L4-100K pAb was a gift from Patrick Hearing (Stony Brook University, New York, USA). The primary anti-penton and anti-fiber rabbit polyclonal serum (prepared by our laboratory) was used at a 1/100 dilution. Secondary antibodies were alkaline phosphatase-conjugated anti-rabbit, anti-mouse, rabbit anti-goat IgG or horseradish peroxidase-conjugated anti-mouse IgG (Jackson Immunoresearch, USA).

The following antibodies were used in this study: anti-HA mouse mAb (Covance, Emeryville, CA, USA), anti-myc mouse mAb (Millipore, Billerica, MA, USA), anti-tubulin mouse mAb (Covance, Princeton, NJ, USA), anti-His mouse mAb (Sigma, St. Louis, MO, USA), anti-Flag mouse mAb (Sigma), anti-BST-2 mouse mAb (Abnova, Taipei, Taiwan), anti-BST-2 rabbit polyclonal antibody (pAb) obtained from the National Institutes of Health, AIDS Research and Reference Reagent Program (NIH-ARRRP, Germantown, MD, USA), and anti-p24 mouse mAb obtained from an HIV-1 p24 hybridoma (NIH-ARRRP).
Alkaline phosphatase conjugated goat anti-rabbit or anti-mouse IgG secondary antibodies were purchased from Jackson Immunoresearch Laboratories (West Grove, PA, USA). Alexa Fluro 488 or 633 goat anti-mouse IgG secondary antibodies were from Invitrogen.

Cells were lysed and protein extracted as described (35 (link)). Proteins were resolved by 8–12% SDS-polyacrylamide gel electrophoresis and electroblotted to nitrocellulose membrane (Bio-Rad). After blocking (5% non-fat dried milk powder in 0.05% Tween-20 in 1xPBS, or 5% BSA in TBST for phospho-proteins) for 40 min, membranes were incubated with rabbit anti-Drosha pAb (Sigma, SAB4200151), mouse anti-GSK3β mAb (Abcam, ab93926), rat anti-AGO2 Ab (gift from Geok Tan, Imperial College London), mouse anti-FUS mAb (SantaCruz, sc-47711), mouse anti-Flag epitope tag M2 mAb (Sigma, F1804), rabbit anti-DGCR8 pAb (Sigma, SAB4200089), rabbit anti-DGCR8 pAb (Abcam ab36865), mouse anti-HA mAb (Covance, 16B12), mouse anti-phospho-serine (Sigma P5747), rabbit anti-PTEN pAb (R+D systems, AF847), rabbit anti-FOXO1 mAb (Abcam, ab52857), rabbit anti-ZEB1 mAb (Cell Signalling, 3396), mouse anti-β-tubulin mAb (Sigma, T4126) or mouse anti-β-actin mAb (Abcam, ab6276) for 1 h and visualised using goat anti-mouse, goat anti-rabbit or goat anti-rat IgG-HRP as appropriate. Detection was by Luminata Forte HRP substrate (Millipore).