Xn 9000 system

Raman spectroscopy We used the Thermo Fisher Scientific DXR SmartRaman Spectrometer (Waltham, Massachusetts, USA) with an excitation wavelength of 780 nm for data collection. Outgoing Raman radiation was detected with a cooled CCD sensor (at -50 • C) in the range from 50 to 3500 cm -1 . After a period of thorough testing and comparing various settings, we finally chose the settings listed in Table S1 in the supplementary material.
A crucial parameter is the duration and the repetition of the measurements taken by the device. Obviously, a higher number of repetitions would be better than a lower number for statistics and, most importantly, for noise cancellation. However, substantial photo-bleaching was observed, especially in range between 1500 and 3000 cm -1 . The device's laser can be focused on different heights. We chose a height such that the background created by the carrier was minimal. The focus was slightly above the level of the coverslips.
Throughout the entire analysis, the dominant background was of obvious concern. Laboratory analysis Serum parameters were measured on cobas 8000 analysers (Roche, Mannheim, Germany). The international normalized ratio (INR) was determined in citrate plasma using the ACL TOP 700 System (Instrumentation Laboratory, Lexington, USA). Blood cells were measured in EDTA whole blood using the XN 9000 system (Sysmex, Kobe, Japan).