Supelco dsc18 cartridges

Optical rotations were measured on a Jasco P-2000 polarimeter (Jasco, Easton, MD, USA). IR spectra were recorded on a Perkin-Elmer FT-IR Spectrum Two spectrometer (Perkin Elmer, Boston, MA, USA). ¹H and ¹³C NMR spectra were recorded on an Agilent 500 (Agilent Technologies, Santa Clara, CA, USA) or on a Bruker 500 spectrometer (Bruker, Billerica, MA, USA) using CD₃OD as solvent. Chemical shifts were referenced using the solvent signals at δ_H 3.30 and δ_C 49.0. COSY, HSQC, HMBC, and NOESY experiments were performed using standard Agilent or Bruker pulse sequences. High resolution mass spectra (HRESIMS) were obtained on a Waters XEVO G2-S Mass spectrometer (Waters, Milford, MA, USA). Column chromatography was carried out on Merck Silica gel 60 (70–230 mesh) (Merck, Darmstadt, Germany). SPE separations were performed on Supelco DSC18 cartridges (500 mg/3 mL or 1 g/6 mL) (Supelco, Bellefonte, PA, USA). HPLC separations were performed on a LaChrom-Hitachi apparatus (Merck, Darmstadt, Germany) using a differential refractometer RI-71. Luna Si (2) (250 × 4.6 mm, 5 μm) (Phenomenex, Torrance, CA, USA) and Luna Si (2) (250 × 10 mm, 5 μm) (Phenomenex, Torrance, CA, USA) columns were used for separations in normal phase. All solvents were of HPLC grade.

Optical rotations were measured on a Jasco P-2000 polarimeter (Jasco, Easton, MD, USA). IR spectra were recorded on a Perkin–Elmer FT-IR Spectrum Two spectrometer (Perkin Elmer, Boston, MA, USA). ¹H and ¹³C NMR spectra were recorded on an Agilent 500 (Agilent Technologies, Santa Clara, CA, USA) or on a Bruker 500 spectrometer (Bruker, Billerica, MA, USA), using CD₃OD as solvent. Chemical shifts were referenced using the solvent signals at δ_H 3.30 and δ_C 49.0. COSY, HSQC, HMBC, and NOESY experiments were performed using standard Agilent or Bruker pulse sequences. High-resolution mass spectra (HRESIMS) were obtained on a Waters XEVO G2-S Mass spectrometer (Waters, Milford, MA, USA). Column chromatography was carried out on Merck Silica gel 60 (70–230 mesh) (Merck, Darmstadt, Germany). SPE separations were performed on Supelco DSC18 cartridges (Supelco, Bellefonte, PA, USA). HPLC separations were performed on a LaChrom-Hitachi apparatus (Merck, Darmstadt, Germany) using a differential refractometer RI-71. Luna Si (2) (250 × 4.6 mm, 5 μm) (Phenomenex, Torrance, CA, USA) and Luna Si (2) (250 × 10 mm, 5 μm) (Phenomenex, Torrance, CA, USA) columns were used for separations in normal phase. All solvents were of HPLC grade.