Cet was obtained from Merck KGaA (Darmstadt, Germany). CisPt was obtained from Boyuan Chemical Company (Shandong, China). Succinic anhydride (SA), 1-isocyanatooctane (C8-NCO), acetone, hydrogen peroxide, ether, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), EDCI, and NHS were obtained from Aladdin (Shanghai, China). Unless otherwise specified, all chemicals were acquired from commercial suppliers and utilized without additional purification. The cell culture vessels were obtained from Corning Inc. (Corning, NY, USA). Dulbecco's modified Eagle's medium (DMEM) containing 4.5 g of glucose, Roswell Park Memorial Institute-1640 (RPMI-1640), trypsin-ethylenediamine tetraacetic acid (EDTA) (0.25%), penicillin/streptomycin, and fetal bovine serum were procured from Gibco (Grand Island, NY, USA). 4',6-diamidino-2-phenylindole (DAPI), propidium iodide (PI), Alexa Fluor® 488, and the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) apoptosis assay kit were acquired from Solarbio Science & Technology Co., Ltd. (Beijing, China). More detailed information about materials and method are shown in the Supplementary data.
4 6 diamidino 2 phenylindole dapi propidium iodide
Manufactured by Solarbio
Sourced in China
4',6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) are fluorescent dyes commonly used in life science research. DAPI is a nuclear stain that binds to double-stranded DNA, emitting blue fluorescence. PI is a nucleic acid intercalating dye that binds to DNA, emitting red fluorescence. These dyes are commonly used for cell visualization and nucleic acid detection applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin ethylenediaminetetraacetic acid edta 0.25, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Cell culture vessels, by Corning (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488, by Solarbio (1 mentions)
Hyaluronic acid, by Merck Group (1 mentions)
Propylene sulfide, by Merck Group (1 mentions)
Dicyclohexylcarbodiimide, by Merck Group (1 mentions)
N boc ethanolamine, by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Thermo Fisher Scientific (1 mentions)
Triethylamine tea, by Thermo Fisher Scientific (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
Adipic dihydrazide, by Merck Group (1 mentions)
Doxorubicin hydrochloride, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Solarbio (1 mentions)
2 7 dichlorofluorescindiacetate dcfh da, by Yeasen (1 mentions)
2 protocols using 4 6 diamidino 2 phenylindole dapi propidium iodide
1
Synthesis and Characterization of Cytotoxic Agents
2
Hyaluronic Acid-based Nanoparticles for Doxorubicin Delivery
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Hyaluronic acid, propylene sulfide, adipic
dihydrazide, caprolactone, N-Boc-ethanolamine, doxorubicin
hydrochloride (DOX·HCl), dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine
(DMAP), ethyl-3-(3-dimethylaminopropyl) carbodiimidehydrochloride
(EDC·HCl), and N-hydroxysuccinimide (NHS) were
purchased from Sigma-Aldrich Co., Ltd. (Beijing, China). N,N-Dimethylformamide (DMF) and triethylamine (TEA)
were purchased from Alfa Aesar Co., Ltd. (Shanghai, China). 2,7-Dichlorofluorescin
diacetate (DCFH-DA) was purchased from Yeasen Biotechnology Co., Ltd.
(Shanghai, China). 4′,6-Diamidino-2-phenylindole (DAPI), propidium
iodide (PI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT), and TUNEL agents were obtained from Solarbio Co., Ltd.
(Beijing, China). Cell culture agents were purchased from GIBCO. Antibodies
were acquired from Abcam Co., Ltd. (Shanghai, China).
dihydrazide, caprolactone, N-Boc-ethanolamine, doxorubicin
hydrochloride (DOX·HCl), dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine
(DMAP), ethyl-3-(3-dimethylaminopropyl) carbodiimidehydrochloride
(EDC·HCl), and N-hydroxysuccinimide (NHS) were
purchased from Sigma-Aldrich Co., Ltd. (Beijing, China). N,N-Dimethylformamide (DMF) and triethylamine (TEA)
were purchased from Alfa Aesar Co., Ltd. (Shanghai, China). 2,7-Dichlorofluorescin
diacetate (DCFH-DA) was purchased from Yeasen Biotechnology Co., Ltd.
(Shanghai, China). 4′,6-Diamidino-2-phenylindole (DAPI), propidium
iodide (PI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT), and TUNEL agents were obtained from Solarbio Co., Ltd.
(Beijing, China). Cell culture agents were purchased from GIBCO. Antibodies
were acquired from Abcam Co., Ltd. (Shanghai, China).
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