Human bladder cancer cell lines (T24 and 5637) and human foreskin fibroblast (HFF) cells were purchased from the Institute of Cell Research, Chinese Academic of Sciences, Shanghai, China. The normal bladder epithelium SV-HUC-1 cell line was established by transformation of human normal ureter tissue with SV40 virus, and purchased from American Type Culture Collection (ATCC). T24 and HFF cells were cultured in DMEM (Invitrogen, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS). The 5637 cells were maintained in 10% FBS RPMI-1640 media (Invitrogen, Carlsbad, CA, USA). The SV-HUC-1 cells were grown according to the manufacturer’s protocol. The cells were cultured at 37 °C in a humidified atmosphere of 5% CO2 in an incubator.
Sv huc 1 cell line
Manufactured by American Type Culture Collection
Sourced in United States
The SV-HUC-1 cell line is a human urothelial cell line derived from normal human urothelial cells. It is immortalized through the introduction of the SV40 large T antigen. The SV-HUC-1 cell line is commonly used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
F 12k medium, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
T24 htb 4, by American Type Culture Collection (1 mentions)
Ribofect cp, by RiboBio (1 mentions)
Polyjet transfection reagent, by SignaGen (1 mentions)
Tccsup, by National Collection of Authenticated Cell Cultures (1 mentions)
Umuc3, by National Collection of Authenticated Cell Cultures (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
Sirna, by GenePharma (1 mentions)
Umuc3, by American Type Culture Collection (1 mentions)
Sw780, by American Type Culture Collection (1 mentions)
5 protocols using sv huc 1 cell line
1
Cultivation of Human Bladder Cell Lines
2
Cell Culture of Human Bladder Cancer Lines
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The human uroepithelial SV-HUC-1 cell line was purchased from the American Type Culture Collection and maintained in F-12K medium (Gibco, Thermo Fisher Scientific, Inc.). The human T24 and 5637 BC cell lines were purchased from the Institute of Cell Biology, Chinese Academy of Sciences (https://www.cellbank.org.cn ). T24 cells were maintained in RPMI-1640, while 5637 cells were maintained in minimum essential medium (both purchased from Gibco; Thermo Fisher Scientific, Inc.). All media were supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) and cultured at 37˚C, with 5% CO2.
3
Culturing Normal and Bladder Cancer Cells
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The normal human bladder epithelial SV-HUC-1 cell line, and human bladder cancer ScaBER (HTB-3), 5637 (HTB-9) and T24 (HTB-4) cell lines were obtained from the American Type Culture Collection and were cultured in Dulbecco's modified Eagle medium (Thermo Fisher Scientific, Inc.), to which 10% fetal bovine serum (Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Inc.) were added.
4
Bladder Cancer Cell Lines Protocol
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Human normal urothelial SV-HUC-1 cells and human bladder cancer cell lines (RT4, T24, J82, UM-UC-3, and TCCSUP) were used. The SV-HUC-1 cell line was obtained from American Type Culture Collection (ATCC), and the RT4, T24, J82, UM-UC-3, and TCCSUP cell lines were obtained from the National Collection of Authenticated Cell Cultures. SV-HUC-1 cells were cultured in F-12K medium (21127022, Gibco, New York, USA) supplemented with 10% fetal bovine serum (FBS; 1750114, Gibco). J82 and TCCSUP cells were cultured in Minimum Essential Medium (MEM) (11095080, Gibco) containing 10% FBS. T24 cells were cultured in DMEM-F12 medium (10565018, Gibco) containing 5% FBS. UM-UC-3 and RT4 cells were cultured in DMEM (11995065, Gibco) containing 10% FBS. The cells were cultured in a humid environment (5% CO2 and 95% O2) at 37°C. Plasmids were transfected in vitro using PolyJet Transfection Reagent (SignaGen Laboratories, Gaithersburg, MD, USA) according to the manufacturer's instructions, and RiboFect CP (riboBio, Guangzhou, China) was used to transfect siRNA (GenePharma, Shanghai, China). All of the siRNA sequences are shown in Table S1 .
5
Cell Line Culturing Protocol
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Human BC (5637, T24, J82, UM-UC-3 and SW780) cell lines and the SV-HUC-1 cell line were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Complete growth medium of these six cell lines was chosen according to ATCC suggestions. Cells were cultured at 37°C supplied with 5% CO2 in an incubator.
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