Luria-Bertani (LB) agar, LB broth and tryptone were purchased from Lab M Limited (Lancashire, UK). Mueller Hinton agar, Mueller Hinton broth, and tryptone soya agar and tryptone soya broth were obtained from Oxoid (Hampshire, UK). Other chemicals were of pharmaceutical grade. Bacterial strains of Pseudomonas aeruginosa PAO1 were kindly donated by the Department of Microbiology, Faculty of Pharmacy, Mansoura University, Egypt.
Lb broth
Manufactured by Lab M
Sourced in United Kingdom
LB broth is a nutrient-rich media commonly used in microbiology for the growth and cultivation of a wide range of bacterial species. It provides essential nutrients required for bacterial proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Luria bertani agar, by Lab M (2 mentions)
Mueller hinton broth (mhb), by Thermo Fisher Scientific (1 mentions)
Mueller hinton agar (mha), by Thermo Fisher Scientific (1 mentions)
Tryptone soya agar, by Thermo Fisher Scientific (1 mentions)
Tryptone soya broth (tsb), by Thermo Fisher Scientific (1 mentions)
Tryptone, by Lab M (1 mentions)
Lb agar, by Lab M (1 mentions)
Kanamycin, by Lab M (1 mentions)
Glycerol, by Merck Group (1 mentions)
Kanamycin, by Merck Group (1 mentions)
Bhi broth, by Lab M (1 mentions)
Tryptone soy broth (tsb), by Thermo Fisher Scientific (1 mentions)
Sitagliptin, by Merck Group (1 mentions)
Mueller hinton broth, by Thermo Fisher Scientific (1 mentions)
Mueller hinton agar, by Thermo Fisher Scientific (1 mentions)
4 protocols using lb broth
1
Antibiotic Susceptibility of Pseudomonas aeruginosa
2
Cultivation and Preparation of E. coli K12
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A pure culture of E. coli strain K12 was obtained from the culture collection of the Department of Food and Nutritional Sciences, University of Reading. The stock culture was kept at -80°C in 15%
(v/v) glycerol and prior to experiments a sample was transferred onto LB Agar (Lab M limited, UK) agar plates which were incubated at 37 °C overnight. The inoculum was prepared by transferring three isolated colonies from the latter plates into 200 ml sterile LB Broth (Lab M limited, UK) in 500 ml sterile flasks and incubated at 37 °C for 18 h to give approximately 10 9 CFU•ml -1 at stationary phase. Subsequently, cells were harvested by centrifugation, (7000 × g for 5 min) and washed twice in sterile phosphate buffered saline solution (PBS, Oxoid, Basingstoke, UK) before they were resuspended in sterile PBS (pH 7.1) at a concentration of approximately 10 9 CFU•ml -1 (stock suspension).
(v/v) glycerol and prior to experiments a sample was transferred onto LB Agar (Lab M limited, UK) agar plates which were incubated at 37 °C overnight. The inoculum was prepared by transferring three isolated colonies from the latter plates into 200 ml sterile LB Broth (Lab M limited, UK) in 500 ml sterile flasks and incubated at 37 °C for 18 h to give approximately 10 9 CFU•ml -1 at stationary phase. Subsequently, cells were harvested by centrifugation, (7000 × g for 5 min) and washed twice in sterile phosphate buffered saline solution (PBS, Oxoid, Basingstoke, UK) before they were resuspended in sterile PBS (pH 7.1) at a concentration of approximately 10 9 CFU•ml -1 (stock suspension).
3
Culturing Bacterial Strains for Research
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The strains used in this study were obtained from the MTU Cork culture collection. L. monocytogenes LO28, Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) ATCC 49416 and Bacillus subtilis subsp. Spizizenii (B. subtilis) ATCC 6633 were grown aerobically in brain heart infusion (BHI) broth (LabM, Lancashire, UK) at 37 °C. E. coli DH5αlux contains a plasmid with a kanamycin resistance gene and was grown at 37 °C in Luria-Bertani (LB) broth (LabM, Lancashire, UK) supplemented with 100 µg/mL kanamycin (Merck, Darmstadt, Germany). Stocks of all strains were maintained at −20 °C and −80 °C in a final concentration of 40% glycerol (Merck, Darmstadt, Germany).
4
Antimicrobial Susceptibility Testing Protocol
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Mueller Hinton (MH) broth, Mueller Hinton (MH) agar and Tryptone soy broth (TSB) were the products of Oxoid (Hampshire, UK). Luria-Bertani (LB) agar and LB broth were purchased from Lab M Limited (Lancashire, United Kingdom). Sitagliptin was obtained from Sigma-Aldrich (St. Louis, USA). Other used chemicals were of pharmaceutical grade.
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