Pka kinase activity kit

The PKA inhibitor H89 and activator 8-bromo-cAMP were purchased from Beyotime Biotechnology (Cat. #S1643; Beijing, China) and Sigma-Aldrich (Cat. B-5380; St. Louis, MO), respectively. The H1HR inhibitor TF and H1HR agonist was purchased from Abcam (Cambridge, UK). Histamine was purchased from Sigma-Aldrich. The PKC kinase activity kit (ab139437) and PKA kinase activity kit (ab139435) were purchased from Abcam (Cambridge, UK). The primary antibodies used in this study and their working concentrations were listed in Supplementary Table 3. The sequences of primers and siRNAs used in this study were listed in Supplementary Table 4.

Glucose Uptake Assay Kit (Colorimetric, ab136955, Abcam) and PKA Kinase Activity Kit (ab139435, Abcam) were used to measure glucose uptake and PKA activity in HUVECs, respectively, according to the manufacturer's protocols.

Recombinant human BDNF was purchased from PeproTech (Rocky Hill, NJ, USA). FSH (human pituitary) and protein G agarose were from Merck Millipore (Darmstadt, Germany). Hoechst 33342 for DNA staining was obtained from Sigma-Aldrich (Darmstadt, Germany). The following antibodies were used: rabbit polyclonal antibodies against β-actin, FSHR and BDNF (Bioworld Technology, Louis Park, MN, USA); rabbit monoclonal antibodies against CREB and phospho-CREB (Ser133) (Cell signaling Technology, Danvers, MA, USA); rabbit polyclonal antibody against ubiquitin and aromatase, and mouse monoclonal antibody against phosphoserine/threonine/tyrosine (Abcam, Shanghai, CHN). The following kits were employed: Human BDNF ELISA Kit (Boster, Wuhan, CHN); cAMP assay kit (R&D Systems, Inc., Minneapolis, MN, USA); PKA kinase activity kit (Abcam, Shanghai, CHN).