Glycogen staining kit

The liver tissue was removed immediately after the mice were sacrificed, and rinsed with cool saline water. Weigh the liver and calculate the liver index (LI, LI = liver weight/body weight×100%).
In addition, the left lobe of the liver was divided into two portions and stored for Western blotting and polymerase chain reaction (PCR) analyses. Following the partial cutting of the intact liver tissue, it was soaked in 4% polyoxymethylene solution, and subsequently subjected to conventional paraffin embedding. Finally, the liver tissue was cut into 3.5-μm-thick sections. After periodic acid-Schiff (PAS) staining using a glycogen staining kit (Cat number: D004, Nanjing Jiancheng Bioengineering Institute, China), the histological structure of liver tissue was observed under a BX50 microscope (Olympus, Japan). The level of hepatic glycogen was measured using Liver/Muscle glycogen assay kit (Cat number: A043-1-1, Nanjing Jiancheng Bioengineering Institute, China).

MTT and DMSO were purchased from Tiangen Biochemical Technology Co., Ltd. (China). Glucose assay kit and glycogen staining kit and human insulin were purchased from Nanjing Jiancheng Co., Ltd. (China). High-glucose DMEM medium and phenol red-free 1640 medium and TRIzol and Lipofectamine 2000 were purchased from Thermo (USA). The PI3K inhibitor wortmannin S1952 was purchased from Biyuntian Biotechnology Company (China). Fetal bovine serum was purchased from Tianjin TBD Company (China). Trypsin was purchased from Promega (USA). cDNA first-strand synthesis kit and PCR mix and SYBR PCR mix were purchased from Takara (China). Protein extraction kit and BCA protein detection kit were purchased from Biyuntian Biotechnology Company (China).