Donor cells were transfected with Cy3‐labeled miR‐34a for 24 hours and selected by BD influx flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA) before the experiment. Receiving cells were stably labeled with GFP and not transfected with miRNA mimics. The donor cells and receiving cells were washed, trypsinized and mixed together in a ratio of 1:1. After 12 hours of coculture, cells were analyzed by confocal microscopy (LSM710, Zeiss, Jena, Germany) or BD influx flow cytometry (BD Biosciences).
Influx flow cytometry
Manufactured by BD
Sourced in United States, Germany
The BD influx flow cytometry system is a laboratory instrument designed for analyzing and sorting cells or particles in a fluid sample. It uses laser technology and specialized detectors to measure and characterize various properties of individual cells or particles as they pass through the system. The core function of the BD influx is to provide researchers and scientists with a tool for high-performance cell analysis and sorting applications.
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Lab products found in correlation
2 protocols using influx flow cytometry
1
Intercellular miRNA Transfer Dynamics
2
Isolation and Culture of Human CD4+ T Cells
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Human peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood of healthy patients, using Ficoll‐Paque (Biochrom, Berlin, Germany) density gradient centrifugation. Then PBMCs incubated with CD4‐APC antibody (BD Biosciences, CA) for 20 minutes. Human CD4+ T cells were isolated from PBMCs via BD influx flow cytometry (BD Biosciences). CD4+ T cells were cultured in RPMI‐1640 containing 10% FBS supplemented with antibiotics.
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