Techtochrysin

Manufactured by Merck Group

Techtochrysin is a specialized piece of laboratory equipment developed by Merck Group. It is designed to perform precise and controlled chromatographic separations, a fundamental technique in analytical chemistry and biochemistry. The core function of Techtochrysin is to facilitate the separation, identification, and purification of complex mixtures of molecules, such as proteins, nucleic acids, or small organic compounds. This equipment allows researchers to achieve accurate and reproducible results in their analytical processes.

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Lab products found in correlation

2 protocols using techtochrysin

Xanthine Oxidase Inhibition Assay

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XO enzyme (grade I, from bovine milk, approximately 10.4 units per mL) and xanthine substrate were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Allopurinol, febuxostat, quercetin, rosmarinic acid, luteolin, kaempferol, isoferulic acid, genkwanin and techtochrysin (analytical grade) were also obtained from Sigma-Aldrich Co. All other reagents and solvents used in the study were of analytical grade.

Ghallab D.S., Shawky E., Metwally A.M., Celik I., Ibrahim R.S, & Mohyeldin M.M. (2022). Integrated in silico – in vitro strategy for the discovery of potential xanthine oxidase inhibitors from Egyptian propolis and their synergistic effect with allopurinol and febuxostat. RSC Advances, 12(5), 2843-2872.

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Comprehensive Phytochemical Analysis Protocol

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The specific compounds, were: Caffeic acid, CAPE, chrysin, luteolin, daidzein, suberic acid, apigenin, (Alfa Aesar), pinocembrin, isorhamnetin, isosakuranetin, vitexin, orientin, rosmarinic acid, myricetin, vanillin, ursolic acid, hydroxytyrosol, tangeretin, chrysoeriol, betulinic acid, eriodictyol, sakuranetin, naringenin, t-cinnamic acid, genistein, diosmetin, resveratrol, galangin, pinocembrin 7-methyl ether, techtochrysin, (Extrasynthese) rutin, isoferulic acid, ellagic acid, kaempferol, quercetin, corosolic acid, acacetin, diosmin, protocatechuic acid ethyl ester, hesperetin, phloridzin, chlorogenic acid, p-coumaric acid, (±)catechin, maslinic acid, (Sigma Aldrich), rhamnetin, syringic acid, protocatechuic acid, ferulic acid, kaempferide, adipic acid, pinostrobin, gallic acid, pinobanksin (Fluka), naringin, hesperidin, (Acros Organics) pinobanksin-3O-acetate (Interchim Inc), cinnamylidenacetic acid, artepillin C (Wako Chemicals). o-Orselllinaldehyde was purchased from Santa Cruz Biotechnology (USA).
Water, acetonitrile, m Ethanol and formic acid were purchased from Fisher Scientific, UK and they were of LC-MS grade. Ethanol was purchased from Merck, Germany. PTFE filters (0.45 μm) were obtained from Macherey-Nagel, Germany.

Kasiotis K.M., Anastasiadou P., Papadopoulos A, & Machera K. (2017). Revisiting Greek Propolis: Chromatographic Analysis and Antioxidant Activity Study. PLoS ONE, 12(1), e0170077.

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