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3 protocols using sodium diethyldithiocarbamate trihydrate

1

Diazo-resin PAN Membrane Synthesis

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PAN membrane were purchased from Shandong Jinhui film Polytron Technologies Inc. Diazo-resin (DR, Mn = 2500) was synthesized according to a method reported elsewhere38 (link). EDTA, cresol red, ammonia chloride and sodium diethyldithiocarbamate trihydrate were all obtained from Sinopharm group Co. ltd.
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2

Synthesis of Inorganic Nanoparticles

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Sodium diethyldithiocarbamate trihydrate (NaDDTC·3H2O, 99%), sodium molybdate dihydrate (Na2MoO4·2H2O), 1-octadecene (1-ODE, 90%), and 1-dodecanethiol (DDT, C12H25SH, 99.5%) were provided by Sinopharm Chemical Reagent Co., Ltd. Aldrich provided analytical solvents such as methanol, ethanol, acetone, and n-hexane. All solvents and reactants were employed without additional purification.
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3

Comprehensive Antioxidant Assays Protocol

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RNA extraction kit was obtained from Beijing TransGen Co., Ltd., China, while qRT-PCR kit and reverse transcription kit were purchased from Beijing Takara Co., Ltd., China.
Chemical reagents (analytical grade), such as dibasic sodium phosphate (Na2HPO4), ferric chloride, potassium ferricyanide, ascorbic acid (AsA), hydrogen peroxide (H2O2), sodium diethyl dithiocarbamate trihydrate, sodium dihydrogen phosphate (NaH2PO4), polyvinyl pyrrolidone (PVP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxylamine hydrochloride, α-naphthyl amine, p-aminobenzenesulfonic acid, sodium nitrite, hydroxylamine, trichloroacetic acid, thibabituric acid, dithiothreitol, nitro blue tetrazolium, riboflavin, ethylenediaminetetraacetic acid disodium salt (EDTA-Na2), bovine serum albumin, catechin, gallic acid, methionine, dithio nitrobenzoic acid (DTNB), glutathione (GSH), methanol, were obtained from Shanghai Sinopharm Co. Ltd., China.
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